Thin-layer chromatography analysis of lipids extracted from the Licuri mesocarp (Mes) and endocarp (End) with cold hexane. (A) Separation of neutral lipids stained with copper acetate-phosphoric acid. DAG, Diacylglycerol (Rf: 1.3-DAG: 0.31 ± 0.02; 1.2(2.3)-DAG: 0.25 ± 0.02); End, endocarp or kernel; FFA, Free fatty acids (Rf: 0.48 ± 0.03); MAG, Monoacylglycerol (Rf: 0.02 ± 0.01); Mes, Mesocarp; Rs, Reference standards including triolein, oleic acid, diolein, and monoolein; TAG, Triacylglycerol (Rf: 0.88 ± 0.05). (B) Separation of polar lipids stained with thymol-sulfuric acid. End, endocarp; MGDG: monogalactosyl diacylglycerol (Rf: 0.63 ± 0.05); DGDG: digalactosyl diacylglycerol (Rf: 0.31 ± 0.02). (C) Hydrolysis of MGDG and DGDG reference standards by pancreatic lipase-related protein 2 (PLRP2), leading to the formation of monogalactosyl monoacylglycerol (MGMG; Rf: 0.40 ± 0.03) and digalactosyl monoacylglycerol (DGMG; Rf: 0.10 ± 0.02). The samples were obtained according to Sahaka et al. (2021). Original pictures are available in the Supporting Information (Figs. S1, S2 and S3).