Volume 10, Number 5-6, Septembre-Décembre 2003
|Page(s)||400 - 404|
|Published online||15 September 2003|
Effect of water content and temperature on Carica papaya lipase catalyzed esterification and transesterification reactions
SEA OIL, Beaumont Hague, France
2 CIRAD Lipotechnie, TA 40/16, 34398, Montpellier, France 04 67 615519 04 67 3615515
Temperature and water activity (a w) of the reaction medium are two factors that govern enzyme reactions. We studied the influence of these two parameters on the esterification and transesterification activity of Carica papaya lipase in water and solvent free reactions. It was found that over the course of reaction the catalytic activity of C. papaya lipase was dependent on these factors. The best lipase activity for both reactions was at a temperature of 55°C and water activity of 0.22, which corresponds to 2 g of water per 100 g of C. papaya latex.
Key words: temperature / water activity / esterification / transesterification / Carica papaya / crude lipase
© John Libbey Eurotext 2003
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