Impact of DHA supplementation on glucose uptake in rat brain endothelial cells primary culture (RBEC). Glucose uptake ([3H]-3-o-methyl glucose method) and GLUT1 protein expression (Western blotting) were measured in RBEC cultived in basal condition (no PUFA supplementation) or supplemented with 15 μM docosahexaenoic acid (DHA, 22:6 n-3) or arachidonic acid (AA, 20:4 n-6) (Pifferi et al., 2010). Glucose uptake was measured using the non-metabolisable glucose analogue 3-o-methylglucose. Values are means ± SD (n = 4 separate experiments each performed in triplicate). *Statistically different from non-supplemented cells (basal) (P < 0.05).