Volume 23, Numéro 1, January-February 2016
|Nombre de pages||5|
|Section||Dossier: Lipids and Brain / Lipides et cerveau|
|Publié en ligne||17 juin 2015|
Long-chain omega3 polyunsaturated fatty acids and cognition in older people: interaction with APOE genotype
Acides gras oméga-3 à longue chaîne et cognition chez les sujets âgés : interaction avec le génotype APOE
University of Bordeaux, ISPED, 33000
2 INSERM, Centre INSERM U897-Épidemiologie, Biostatistique, 33000 Bordeaux, France
3 Centre de recherche sur le vieillissement du département de médecine, Université de Sherbrooke, Sherbrooke, Québec, Canada
Received: 26 April 2015
Accepted: 11 May 2015
Basic research and epidemiological studies suggest a protective effect of long-chain omega3 polyunsaturated fatty acids (LC n-3 PUFA) against age-related cognitive decline. However, most randomized controlled trials with LC n-3 PUFA supplements have yielded disappointing results on cognitive outcomes in older persons. One explanation for this discrepancy may be an inadequate targeting of potential beneficiaries of LC n-3 PUFA according to their Apolipoprotein E (APOE) genotype. The aim of this paper was to examine the potential modifying effect of APOE genotype on LC n-3 PUFA metabolism and its relation to cognitive decline in older persons. At least five epidemiological studies and three intervention studies with LC n-3 PUFA supplements have found an interaction between LC n-3 PUFA and APOE genotype on cognition. However, the direction of the effect is inconsistent across studies: the impact of LC n-3 PUFA on cognition is stronger in APOE4 carriers (the main genetic risk factor for Alzheimer’s disease) in some studies, but conversely stronger in APOE4 non-carriers in other studies. These discordant results may be explained by different age groups, cognitive status, measures of cognition, or amounts of DHA intake across studies. Experimental studies suggest that the APOE4 genotype modifies the metabolism of DHA. The APOE genotype should be systematically taken into account and interactions tested in epidemiological and intervention studies with LC n-3 PUFA. Further research is needed to better understand the underlying mechanisms of this gene X diet interaction.
La recherche fondamentale et les études épidémiologiques suggèrent un effet protecteur des acides gras oméga3 à longue chaîne (AGPI-LC n-3) contre le déclin cognitif lié à l’âge. Cependant les études d’intervention avec des suppléments d’AGPI-LC n-3 ont donné des résultats décevants sur la cognition. Une raison pourrait en être un ciblage inadéquat des bénéficiaires potentiels d’une supplémentation en raison notamment de leur génotype pour le gène de l’Apolipoprotéine E (APOE). L’objectif de cet article était d’explorer l’effet potentiellement modificateur du génotype de l’APOE sur le métabolisme des AGPI-LC n-3 et sa relation avec le déclin cognitif chez le sujet âgé. Au moins cinq études épidémiologiques et trois études d’intervention ont mis en évidence une interaction entre les AGPI-LC n-3 et le génotype de l’APOE sur la cognition des personnes âgées. Cependant la direction de l’effet est inconstant entre les études, certaines montrent un impact des AGPI-LC n-3 uniquement chez les porteurs de l’allèle epsilon4 (APOE4), principal facteur de risque génétique de maladie d’Alzheimer, et d’autres inversement un effet chez les non-porteurs de l’APOE4. Ces résultats discordants peuvent s’expliquer par des différences d’âge, de statut cognitif, de domaine de la cognition mesuré, ou de quantités de DHA entre études. Les études expérimentales suggèrent que le génotype de l’APOE modifie le métabolisme du DHA. Les études épidémiologiques et d’intervention avec des AGPI-LC n-3 devraient systématiquement rechercher l’existence d’une interaction éventuelle avec l’APOE. La recherche doit se poursuivre pour comprendre les mécanismes sous-jacents à cette interaction gène X nutrition.
Key words: Cognition / aging / omega3 fatty acids / Apolipoprotein E gene / DHA
Mots clés : Cognition / vieillissement / acides gras oméga3 / gène de l’Apolipoprotéine E / DHA
© P. Barberger-Gateau et al., published by EDP Sciences, 2015
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Alzheimer’s disease (AD) is the main cause of cognitive decline and dementia in older persons (Cummings, 2004). AD is characterized by the progressive accumulation of beta-amyloide (Abeta) peptide in senile plaques and hyperphosphorylated tau protein in neurofibrillary tangles. These neurodegenerative lesions are accompanied by increased oxidative stress, inflammation, and neuronal death leading to brain atrophy and cognitive decline (Querfurth and LaFerla, 2010).
AD results from a complex interplay between non modifiable risk factors, such as age and genetics, especially the epsilon 4 allele of the Apolipoprotein E gene (APOE4), and modifiable protective or detrimental factors (Barberger-Gateau, Lambert, et al., 2013). In particular, high blood cholesterol at midlife has been associated with increased risk of late-life cognitive decline and dementia (Solomon et al., 2009). High blood cholesterol is a well known vascular risk factor but it may also impair the brain-blood barrier (Shobab et al., 2005). In the brain, cholesterol is an important component of neuron membranes and regulates the scission of the amyloid precursor protein (APP) to Abeta (Shobab et al., 2005).
Among protective factors, nutrition is one of the most promising avenues for the prevention of AD (Barberger Gateau, Lambert, et al., 2013). In particular, basic research as well as epidemiological studies suggest a protective effect of long-chain omega3 polyunsaturated fatty acids (LC n-3 PUFA) against pathological brain aging and cognitive decline (Barberger Gateau, Feart, et al., 2011; Barberger Gateau, Samieri, et al., 2013; Cunnane et al., 2009). However, most randomized controlled trials (RCT) with LC n-3 PUFA supplements have been disappointing regarding cognitive outcomes in older persons (Jiao et al., 2014). One explanation for this discrepancy may be an inadequate targeting of potential beneficiaries of LC n-3 PUFA according to their APOE genotype (Barberger Gateau, Samieri, et al., 2013).
Indeed, the APOE genotype might modify the impact of n-3 on cognition in AD (Barberger Gateau, Samieri, et al., 2011). The aim of this narrative review was to examine the potential modifying effect of APOE genotype on LC n-3 PUFA metabolism and its relation to AD and cognitive decline in older persons.
The APOE gene located on chromosome 19 has three different alleles: ϵ2, ϵ3 and ϵ4. The risk of late-onset AD is strongly increased in individuals carrying the ϵ4 allele (APOE4): this risk is increased by about 14 fold in homozygotes and by 3 fold in heterozygotes (Farrer et al., 1997; Genin et al., 2011). Conversely, the risk of AD is decreased in APOE2 carriers. This translates into a cumulative risk of AD at age 85 of about 50% in homozygotes, 25% in heterozygotes, vs. only 10% in non carriers of the APOE4. In European countries, about one in five individuals carries at least one ϵ4 allele, with some variability between populations.
The APOE gene encodes for apoliprotein E (ApoE). ApoE3 is the most common isoform. ApoE is a cholesterol transporter: the ApoE4 isoform binds preferably to LDL and VLDL cholesterol while ApoE2 and ApoE3 bind preferably to HDL cholesterol (Lane and Farlow, 2005). ApoE isoforms influence the composition of membrane lipid rafts and delivery of essential fatty acids such as DHA to neurons (Lane and Farlow, 2005). In particular, ApoE can coordinate the mobilization of cholesterol for maintenance and repair of neuron membranes (Poirier, 2003). In addition to its role in lipid metabolism, ApoE exerts multiple functions (Jofre-Monseny et al., 2008). Indeed, ApoE has antioxidant properties depending on its isoforms, with a protective effect of ApoE2 while ApoE4 is associated with increased oxidative stress. ApoE modulates inflammation in as isoform dependent manner, especially in the brain where ApoE is associated with increased microglial activation resulting in greater inflammation (Lane and Farlow, 2005). ApoE4 is also associated with decreased cerebral glucose metabolism (Lane and Farlow, 2005). Finally, ApoE binds to soluble Abeta in an isoform dependent pattern thus contributing to its clearance from both extra-cellular and intra-cellular compartments in the brain, ApoE4 being associated with less efficient clearance of Abeta at the blood-brain barrier (Lambert and Amouyel, 2011; Poirier, 2003). All these mechanisms explain why the ApoE4 isoform is associated with an increased risk of late-onset AD.
Epidemiological studies showing an interaction between LC n-3 PUFA and APOE genotype on cognition.
Several epidemiological and intervention studies, but not all, have found an interaction between LC n-3 PUFA intake or blood levels and APOE4 on cognition. Table 1 summarizes the few epidemiological studies that have tested and evidenced this interaction. The first two studies (Barberger-Gateau et al., 2007; Huang et al., 2005) have examined the association between fish consumption and incidence of dementia. Both have found that the risk of dementia was decreased by 40% by regular fish consumption only in APOE4 non-carriers while no association was observed in APOE4 carriers. The two following studies (Beydoun et al., 2007; Whalley et al., 2008) have considered the relationship between blood concentrations of EPA and DHA and cognitive decline. Similarly, both have observed a significantly lower decline with higher proportions of EPA and DHA only in APOE4 non-carriers.
The last study conducted in a sample of 1214 elderly community dwellers participating in the Three-City (3C) study in Bordeaux has also linked blood EPA and DHA to cognitive decline but has yielded conflicting results (Samieri et al., 2011). Indeed, in APOE4 non-carriers, there was no significant association between plasma DHA or EPA and cognitive decline over 7 years on Benton Visual Retention Test (BVRT), a test of working memory and attention, except a protective association with EPA in subjects with high depressive symptoms. Conversely, a protective association was observed for both EPA and DHA with performance on BVRT in APOE4 carriers. There was no association of plasma EPA or DHA with Isaac Set Test, Trail Making Test or Mini Mental Status Examination, a test of global cognitive functioning, whatever the APOE genotype.
Three intervention studies with EPA and/or DHA have also found an interaction with APOE4 genotype. In the first study, healthy young adults consuming very little fish (less than 200 mg EPA plus DHA per week) were randomized to receive 1.16 g DHA per day or a placebo (Stonehouse et al., 2013). Analyses were stratified according to APOE genotype. Both APOE4 carriers and non-carriers who received DHA showed improvements in working memory but the effect was considerably greater in APOE4 carriers due to both improvements in the DHA group and a worsening of performance in the placebo group. There was no significant improvement of attention with DHA. However, APOE4 carriers who received DHA did not worsen as did those receiving the placebo hence the difference was significant. Thus this study seemed to show a higher impact of DHA in APOE4 carriers.
A large RCT comparing two different doses of EPA and DHA and a placebo in 302 cognitively healthy older individuals did not find any significant effect of the supplementation on cognition, except an improvement in the cognitive domain of attention in APOE4 carriers with both doses of supplementation compared to placebo (van de Rest et al., 2008). However, this finding might be due to chance because of multiple comparisons in that study.
The third study included 402 individuals who already had mild to moderate AD, consuming little fish (<200 mg/d DHA) (Quinn et al., 2010). They were randomized to receive 2 g/d DHA or a placebo for 18 months. There was no impact of DHA on any of the cognitive outcomes or MRI biomarkers. However, in post-hoc analyses stratified according to APOE4 genotype, there was a significantly slower decline on ADAS Cog, assessing global cognitive functions, in APOE4 non-carriers receiving DHA, contrarily to what was observed in the other two intervention studies.
In summary, several studies have reported this APOE gene X diet interaction, with inconsistent results: the impact of LC n-3 PUFA on cognition is more important in APOE4 carriers in some studies, in APOE4 non-carriers in other studies. One of the plausible biological explanations for this interaction might be that APOE genotype modifies the metabolism of long-chain n-3 PUFA.
Relationship between frequency of fish intake and plasma DHA according to APOE4 genotype. The Three-City study Bordeaux.
Very few studies have investigated this hypothesis. In the 3C study we examined the relationship between fish consumption and plasma DHA according to APOE4 genotype in elderly community dwellers and found a borderline significant interaction (P = 0.06) (Samieri et al., 2013). In individuals eating little fish, that is less than one serving per week, carriers of the APOE4 had higher mean plasma DHA than non carriers (Fig. 1). There was no significant difference in plasma DHA between carriers and non-carriers for higher frequency of fish intake. Plasma DHA strongly increased with increasing fish intake in both groups. There was no interaction with APOE genotype for plasma EPA.
The group of S.C. Cunnane has further investigated this issue in a series of experimental studies. The first study included 28 adult men who were given a supplement containing 3 g/d EPA+DHA for 6 weeks (Plourde et al., 2009). The proportion of fatty acids in each plasma fraction was measured before and after supplementation. At baseline, APOE4 carriers had higher EPA proportions in non-esterified fatty acids than non-carriers but this proportion did not increase during the supplementation (P for interaction 0.04). There was no significant difference between APOE4 carriers and non-carriers for EPA in triglycerides but the increase was slightly less pronounced in APOE4 carriers (borderline significant P = 0.07 for interaction). Regarding DHA, there was no significant difference between carriers and non-carriers for change in non-esterified fatty acids although the proportion of DHA was always slightly lower in APOE4 carriers. Conversely, the proportion of DHA in triglycerides was slightly higher at baseline but increased less in APOE4 carriers than non-carriers after supplementation. Thus APOE4 carriers appeared to be less responsive to supplementation with LC n-3 PUFA, suggesting an altered n-3 metabolism.
The second experiment involved 40 cognitively healthy older participants who received a single dose of 40 mg DHA labeled with carbon 13 ([13C]-DHA) (Chouinard-Watkins et al., 2013). In APOE4 carriers, [13C]-DHA in plasma total lipids from 1 h to 28 days post-dose was 31% lower compared with APOE4 non-carriers. APOE4 carriers also had higher cumulative beta-oxidation of [13C]-DHA which could explain their lower plasma [13C]-DHA. There was no difference between APOE4 carriers and non-carriers for apparent retroconversion of [13C]-DHA into [13C]-EPA (Chouinard-Watkins et al., 2013). This disturbance in DHA metabolism in APOE4 carriers might contribute to their greater vulnerability to cognitive decline.
In the last experiment, the [13C]-DHA tracer was administered again after a period of 5 months of supplementation with 3.2 g/d of EPA+DHA (Hennebelle et al., 2014). During the supplementation, beta-oxidation of [13C]DHA was lower in APOE4 carriers contrarily to what was observed before supplementation (Chouinard-Watkins and Plourde, 2014). Moreover, APOE4 carriers had an increased plasma response to the tracer after the supplementation, suggesting slower clearance of DHA to or use by tissues (Hennebelle et al., 2014).
At least five epidemiological studies and three intervention studies with LC n-3 PUFA supplements have found an interaction between LC n-3 PUFA and APOE genotype on cognition. However, the direction of the effect is inconsistent across studies: the impact of LC n-3 PUFA on cognition is stronger in APOE4 non-carriers in some studies, but conversely stronger in APOE4 carriers in other studies. These discordant results may be explained by different age groups (adults vs. older persons), cognitive status (healthy vs. mild Alzheimer’s disease), measures of cognition (working memory, attention, or global cognition) or amounts of DHA intake across studies. These inconsistent findings require that the APOE genotype should be systematically taken into account and interactions tested in epidemiological and intervention studies with LC n-3 PUFA.
Experimental studies reported in this paper suggest that the APOE4 genotype modifies the metabolism of DHA. Other studies have shown that APOE genotype may also affect the impact of LC n-3 PUFA on inflammation, triglycerides concentration or cholesterol level (Anil, 2007; Carvalho-Wells et al., 2010; Carvalho-Wells et al., 2012). Further research is needed to better understand the underlying mechanisms of this gene X diet interaction.
P. Barberger-Gateau reports grants and non-financial support from Danone Research, Vifor Pharma and Groupe Lipides et Nutrition, personal fees and non-financial support from Nutricia, ILSI Europe and PiLeJe, and a grant from CNIEL (Centre National Interprofessionnel de l’Industrie Laitière).
- Anil E. 2007. The impact of EPA and DHA on blood lipids and lipoprotein metabolism: influence of apoE genotype. Proc. Nutr. Soc. 66: 60–68. [CrossRef] [PubMed] [Google Scholar]
- Barberger-Gateau P, Lambert JC, Feart C, et al. 2013. From genetics to dietetics: the contribution of epidemiology to understanding Alzheimer’s disease. J. Alzheimers. Dis. 33: S457–S463. [PubMed] [Google Scholar]
- Barberger Gateau P, Feart C, Samieri C, Letenneur L. Dietary patterns and dementia. In: Chronic Medical Disease and Cognitive Aging: Toward a Healthy Body and Brain, Yaffe K, ed. New York, Oxford University Press, 2013. [Google Scholar]
- Barberger Gateau P, Samieri C, Feart C, Cunnane S. 2013. Acides gras oméga-3 et déclin cognitif : la controverse. OCL 20: 88–92. [CrossRef] [EDP Sciences] [Google Scholar]
- Barberger Gateau P, Feart C, Samieri C. 2011. Mediterranean diet and cognitive decline: what role for omega-3 polyunsaturated fatty acids? OCL 18: 224–227. [EDP Sciences] [Google Scholar]
- Barberger Gateau P, Samieri C, Feart C, Plourde M. 2011. Dietary omega 3 polyunsaturated fatty acids and Alzheimer’s disease: Interaction with apolipoprotein E genotype. Curr. Alzheimer. Res. 8: 479–491. [Google Scholar]
- Barberger-Gateau P, Raffaitin C, Letenneur L, et al. 2007. Dietary patterns and risk of dementia: the Three-City cohort study. Neurology 69: 1921–1930. [CrossRef] [PubMed] [Google Scholar]
- Beydoun MA, Kaufman JS, Satia JA, Rosamond W, Folsom AR. 2007. Plasma n-3 fatty acids and the risk of cognitive decline in older adults: the Atherosclerosis Risk in Communities Study. Am. J. Clin Nutr. 85: 1103–1111. [PubMed] [Google Scholar]
- Carvalho-Wells AL, Jackson KG, Lockyer S, Lovegrove JA, Minihane AM. 2012. APOE genotype influences triglyceride and C-reactive protein responses to altered dietary fat intake in UK adults. Am. J. Clin. Nutr. 96: 1447–1453. [CrossRef] [PubMed] [Google Scholar]
- Carvalho-Wells AL, Jackson KG, Gill R, et al. 2010. Interactions between age and apoE genotype on fasting and postprandial triglycerides levels. Atherosclerosis 212: 481–487. [Google Scholar]
- Chouinard-Watkins R, Plourde M. 2014. Fatty acid metabolism in carriers of apolipoprotein E epsilon 4 allele: is it contributing to higher risk of cognitive decline and coronary heart disease? Nutrients 6: 4452–4471. [CrossRef] [PubMed] [Google Scholar]
- Chouinard-Watkins R, Rioux-Perreault C, Fortier M, et al. 2013. Disturbance in uniformly 13C-labelled DHA metabolism in elderly human subjects carrying the apoE epsilon4 allele. Br. J. Nutr. 1–9. [Google Scholar]
- Cummings JL. 2004. Alzheimer’s Disease. N. Engl. J. Med. 351: 56–67. [CrossRef] [PubMed] [Google Scholar]
- Cunnane SC, Plourde M, Pifferi F, Bégin M, Féart C, Barberger-Gateau P. 2009. Fish, docosahexaenoic acid and Alzheimer’s disease. Prog. Lipid Res. 48: 239–256. [CrossRef] [Google Scholar]
- Farrer LAP, Cupples LAP, Haines JLP, et al. 1997. Effects of age, sex, and ethnicity on the association between apolipoprotein E genotype and Alzheimer disease: a meta-analysis. JAMA 278: 1349–1356. [Google Scholar]
- Genin E, Hannequin D, Wallon D, et al. 2011. APOE and Alzheimer disease: a major gene with semi-dominant inheritance. Mol. Psychiatry 16: 903–907. [CrossRef] [PubMed] [Google Scholar]
- Hennebelle M, Plourde M, Chouinard-Watkins R, Castellano CA, Barberger-Gateau P, Cunnane SC. 2014. Ageing and apoE change DHA homeostasis: relevance to age-related cognitive decline. Proc. Nutr. Soc. 73: 80–86. [CrossRef] [PubMed] [Google Scholar]
- Huang TL, Zandi PP, Tucker KL, et al. 2005. Benefits of fatty fish on dementia risk are stronger for those without APOE epsilon 4. Neurology 65: 1409–1414. [CrossRef] [PubMed] [Google Scholar]
- Jiao J, Li Q, Chu J, Zeng W, Yang M, Zhu S. 2014. Effect of n-3 PUFA supplementation on cognitive function throughout the life span from infancy to old age: a systematic review and meta-analysis of randomized controlled trials. Am. J. Clin. Nutr. 100: 1422–1436. [CrossRef] [PubMed] [Google Scholar]
- Jofre-Monseny L, Minihane AM, Rimbach G. 2008. Impact of apoE genotype on oxidative stress, inflammation and disease risk. Mol. Nutr. Food Res. 52: 131–145. [Google Scholar]
- Lambert J-C, Amouyel P. 2011. Genetics of Alzheimer’s disease: new evidences for an old hypothesis? Curr. Opin. Genet. Dev. 21: 295–301. [CrossRef] [PubMed] [Google Scholar]
- Lane RM, Farlow MR. 2005. Lipid homeostasis and apolipoprotein E in the development and progression of Alzheimer’s disease. J. Lipid Res. 46: 949–968. [Google Scholar]
- Plourde M, Vohl M-C, Vandal M, Couture P, Lemieux S, Cunnane SC. 2009. Plasma n-3 fatty acid response to an n-3 fatty acid supplement is modulated by apoE 4 but not by the common PPAR-alpha L162V polymorphism in men. Br. J. Nutr. 102: 1121–1124. [CrossRef] [PubMed] [Google Scholar]
- Poirier J. 2003. Apolipoprotein E and cholesterol metabolism in the pathogenesis and treatment of Alzheimer’s disease. Trends Mol. Med. 9: 94–101. [CrossRef] [PubMed] [Google Scholar]
- Querfurth HW, LaFerla FM. 2010. Alzheimer’s Disease. N. Engl. J. Med. 362: 329–344. [Google Scholar]
- Quinn JF, Raman R, Thomas RG, et al. 2010. Docosahexaenoic acid supplementation and cognitive decline in Alzheimer disease: a randomized trial. JAMA 304: 1903–1911. [CrossRef] [PubMed] [Google Scholar]
- Samieri C, Lorrain S, Buaud B, et al. 2013. Relationship between diet and plasma long-chain n-3 PUFA in older people:Impact of Apolipoprotein E genotype. J. Lipid Res. 54: 2559–2567. [CrossRef] [PubMed] [Google Scholar]
- Samieri C, Feart C, Proust-Lima C, et al. 2011. Omega-3 fatty acids and cognitive decline: modulation by ApoEepsilon4 allele and depression. Neurobiol. Aging. 32: 2317.e13–22. [Google Scholar]
- Shobab LA, Hsiung G-YR, Feldman HH. 2005. Cholesterol in Alzheimer’s disease. Lancet. Neurol. 4: 841–852. [Google Scholar]
- Solomon A, Kivipelto M, Wolozin B, Zhou J, Whitmer RA. 2009. Midlife serum cholesterol and increased risk of Alzheimer’s and vascular dementia three decades later. Dement. Geriatr. Cogn. Disord. 28: 75–80. [CrossRef] [PubMed] [Google Scholar]
- Stonehouse W, Conlon CA, Podd J, et al. 2013. DHA supplementation improved both memory and reaction time in healthy young adults: a randomized controlled trial. Am. J. Clin. Nutr. 97: 1134–1143. [CrossRef] [PubMed] [Google Scholar]
- Van de Rest O, Geleijnse JM, Kok FJP, et al. 2008. Effect of fish oil on cognitive performance in older subjects: A randomized, controlled trial. Neurology 71: 430–438. [CrossRef] [PubMed] [Google Scholar]
- Whalley LJ, Deary IJ, Starr JM, et al. 2008. n-3 Fatty acid erythrocyte membrane content, APOE epsilon4, and cognitive variation: an observational follow-up study in late adulthood. Am. J. Clin. Nutr. 87: 449–454. [PubMed] [Google Scholar]
Cite this article as: Pascale Barberger-Gateau, Cécilia Samieri, Stephen C. Cunnane. Long-chain omega3 polyunsaturated fatty acids and cognition in older people: interaction with APOE genotype. OCL 2016, 23(1) D111.
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