Genetic relationships between three new P. macdonaldii isolates PM1, PM2, PM3 and related strains retrieved from GenBank. Neighbor-joining tree was obtained based on the phylogenetic analysis of the ITS region including ITS1, 5.8 s and ITS2 by using MEGA 6.0. Ten sequences of related strains were retrieved by Blast searches. Bootstrap values above 50% were indicated (1000 replicates). The ascomycete fungi Sclerotinia sclerotiorum was used as the outgroup strain.