Synthesis of different PA species during neurosecretion and neurite outgrowth. PC12 cells were either treated with 10 μM of NGF for 3 days to stimulate neurite outgrowth (Ammar et al., 2013a,b) or with a 59 mM potassium solution for 10-minutes to stimulate catecholamine neurosecretion (Béglé et al., 2009). Cells were washed with ice cold PBS, before being lyzed as described previously (Vitale et al., 2002). Proteins levels were quantified and levels of 40 different PA species were estimated in 200 μg fractions by lipidomic analysis (Kassas et al., 2017). The levels of the 10 most abundant PA species are shown and expressed as pmol/200 μg of proteins. The total carbon chain length and saturation is indicated for each PA species. Results were obtained from 3 independent experiments (^*p < 0.001).