Rod calcium currents (I_Ca) and rod bipolar cell (RBC) glutamate receptor signaling were similar between 12-month-old WT and KO mice. (A) Representative I_Ca recorded with a voltage ramp (–90 to +60, 0.5 mV/ms) in a rod from a WT retina. The displayed trace is an average of two traces from a single cell. (B) I_Ca from a KO rod. The displayed trace is an average of three traces from a single cell. (C) Left: group data, showing that the I_Ca amplitude was similar in WT and KO rods. Likewise, the voltage dependence, as indicated by the peak voltage (V_peak) and voltage of half-maximal activation (V₅₀), was similar in rods from WT and KO mouse retinas. (D) Response of an RBC from a WT retina to a 1-second puff of CPPG (600 μM) in the presence of L-AP4 (4 μM). The RBC was voltage clamped at –60 mV, and the displayed trace is an average of five traces. (E) Response of an RBC from a KO retina. The displayed trace is an average of five traces. (F) Amplitudes of RBC responses to CPPG puffs were similar in WT and Elovl4 KO mouse retinas. Data are mean ± SEM. ns, not significant. (This research was originally published in Investigative Ophthalmology and Visual Science. Bennett et al. (2014). © Association for Research in Vision and Ophthalmology.)