Reduced VLC-PUFAs caused synaptic terminals to mislocalize in mouse retinas. (A) Glutamatergic vesicles labeled with VGlut1 (green) preferentially localized to the OPL in the WT mice but were found predominantly in the ONL of the KO mice. Bipolar dendrites labeled with PKCα (red) in the WT and KO mice. Scale bars: 20 μm. VLC-PUFA–deficient mice had a loss of photoreceptors and synaptic reorganization. (B) Vesicles labeled with VGlut1 (green) were localized with the bipolar cell dendrites labeled with PCK-α (orange) in the photoreceptor synaptic layer (OPL) in the WT, but were found in the ONL and OPL in the KO retina. The OPL in the KO retina was disorganized compared to WT with bipolar cell dendrites (orange) extending down into the ONL. Similar results were observed from eight different 9-month-old mice per genotype. Scale bars: 10 μm. (This research was originally published in Investigative Ophthalmology and Visual Science. Bennett et al. (2014). © Association for Research in Vision and Ophthalmology.)