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Home All issues Volume 26 (2019) OCL, 26 (2019) 14 Full HTML
Open Access
Review
Issue
OCL
Volume 26, 2019
Article Number 14
Number of page(s) 7
Section Technology
DOI https://doi.org/10.1051/ocl/2019006
Published online 29 March 2019

© M. Sharma et al., Published by EDP Sciences, 2019

Licence Creative CommonsThis is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

1 Introduction

Flaxseed also called linseed (Linum usitatissimum) is a major oilseed crop belonging to Lineaceae family. Flax grows in various regions of the world such as India, Argentina, America, China, Canada, etc. Among these countries, the world’s largest exporter of flaxseed is Canada accounting for 75% of global flaxseed trade and also produces 40% of entire world’s flaxseed (Pradhan et al., 2009). Flaxseed is a nutrition booster and plays an important role in increasing the quality of food (Marpalle et al., 2014). A studies suggested that addition of roasted flaxseed in bread led to increase in the calorie content, low glycemic index and high antioxidant activity (Marpalle et al., 2015). Every part of the plant can either be used directly or can be utilized in processed form. The stem of flax contains the best quality fiber having strength and durability. Flaxseed is also rich in its nutritional value. The seeds of flax are rich in oil having omega-3 fatty acids, proteins and lignans (Singh et al., 2011). Flaxseed constitutes 40–45% lipids, 20–25% protein, 20–25% fiber and 1% lignans (Tirgar et al., 2016). The flaxseed is mainly utilized for the industrial purposes such as manufacturing of paint, inks, cosmetic products and varnishes. Flaxseed is the main focus of the researchers due to its various health benefits by its prime biological active components such as alpha-linolenic acid, lignan-secoisolariciresinol diglycoside (SDG) and dietary fibers (Touré and Xueming, 2010). In last few decades flaxseed, an underutilized oilseed has gained importance as functional foods due to its unique nutrient profile (Kaur et al., 2017). The increased health concerns of saturated fats have led to the utilization of omega-3 polyunsaturated acids (Khattab and Zeitoun, 2013). Flaxseed oil possess increased amount of polyunsaturated fatty acids and less saturated fatty acids. However, this property also makes it receptive to the oxidative rancidity that reduces the shelf life of flaxseed oil. Therefore, flax oil is not preferred for cooking (Malcolmson et al., 2000). Flaxseed is rich in lignans among which SDG is the main lignan and possess health benefits (Kaur et al., 2018). It has anti-carcinogenic and antioxidant effects. With the action of bacterial flora, it gets digested in the colon to produce mammalian lignans and protects the human from breast and prostate cancer (Zhang and Xu, 2007). Flaxseed proteins are useful for the immune system. They are rich in arginine and glutamine, so the keep the heart healthy. Flaxseed proteins also have a fungistatic effect against the plant and human pathogens (Tehrani et al., 2014). Due to these potential health benefits of different flaxseed components, there is a necessity for the development of innovative extraction techniques. Various methods like phase extraction, enzyme-assisted extractions and supercritical extraction are in practice. The newer techniques should give high yield, consume less energy and time and be eco-friendly with less use of the chemicals. Traditional methods used for extraction were time and energy consuming. Due to the use of different solvents, there were high risks of different health hazards. This review summarizes different novel techniques which can be used as an alternative to conventional methods. The different techniques opted for extraction of oil, lignans, phenolic acids and protein are reported.

2 Nutritional composition

Among all the functional foods, flaxseed is a great source of ALA, lignans, proteins and phenolic compounds (Oomah, 2001). Growing environment and genetic conditions decide the chemical composition of flaxseed. Flaxseed is rich in oil content which contributes about 98% triacylglycerol, phospholipids and 0.1% of fatty acids (Kajla et al., 2015). Flaxseed contains 20–25% proteins. Globulin (58–66%) and albumin (20–42%) constitutes the major protein fractions (Rabetafika et al., 2011). Flaxseed contains high amount of lignans (phytoestrogens) and phenolic compounds. The major lignan present in flaxseed is secoisolariciresinol diglycoside (SDG) which accounts for 77 to 209 mg SDG/tbsp of whole flaxseed (Morris, 2007). SDG is 11.7 to 24.1 mg/g and 6.1 to 13.3 mg/g in defatted and whole seed flour respectively. Flaxseeds also contain anti-nutrients that cause adverse influence on the health of human beings. Cyanogenic glycosides are the major anti-nutrients and are fractionated into linustatin (213–352 mg/100 g), neolinustatin (91–203 mg/100 g) and linmarin (32 mg/100 g). The content of these three glycosides depend upon cultivar and location. Phytic acid is another anti-nutrient present in flaxseed that ranges from 23 to 33 g/kg of the flaxseed meal. Phytic acid hinders the absorption of calcium, copper, magnesium, etc. The presence of trypsin inhibitors is also reported in flaxseeds. Flaxseed have a unique place among the oilseeds because of the presence of mucilage located in outer layers of the seed (Singh et al., 2011). Flaxseed mucilage has many health benefits and potential functional properties. Flaxseed mucilage increases the viscosity of intestinal contents and delays gastric emptying and nutrient absorption (Kajla et al., 2015). Flaxseed has large amount of phenolic acids that is 800–1000 mg per 100 g of the seeds (Herchi et al., 2014). The main phenolic acids that are present in defatted sample of the flaxseed are gallic acid (2.8 mg/g), chlorogenic acid (7.5 mg/g) and ferulic acid (10.9 mg/g). Other phenolics are present in very less quantities which includes p-coumaric acid glucosides, hydroxycinnamic acid glucosides and 4-hydroxybenzoic acid. Flaxseed is a great source of minerals mainly potassium (650 mg/100 g), magnesium (350–431 mg/100 g) and calcium (236–250 mg/100 g) (Morris, 2007).

3 Different techniques for the extraction of the flaxseed oil

Extraction of oils from oilseeds is a key step for their commercialization. The extraction process directly affects the quantity and quality of oils. Traditionally, oil is extracted by mechanical screw pressing or by solvent extraction using hexane, involves enormous construction and operational costs. The conventional techniques of solvent extraction give a dark coloured meal which is thought to be due to the interaction of the protein with polyphenols. It is envisaged that the new extraction technologies would not only allow complete utilization of flaxseed but also give a quality product. The traditional methods are time and energy consuming, associated with high safety hazard, greater energy input, inferior quality oil, low quality meal, environmental risk and toxicological effects. All these drawbacks associated with hexane-extraction have urged the edible oil industry to seek alternative techniques for extraction of oils which are suitable and environment friendly. Flaxseed oil is commonly utilized as nutraceutical due to the presence of polyunsaturated fatty acids. Existence of omega-3 fatty acids in oil increases its nutritional value. Flaxseed oil is also utilized for various industrial purposes such as for the manufacturing of varnishes, paints, cosmetic products, putty and finishing of wood. Flaxseed oil can be extracted from various techniques. Table 1 represents the different techniques that are used for the extrication of oil from flaxseeds.

Table 1

Different techniques for the extraction of oil from flaxseed.

3.1 Supercritical fluid CO2-extraction

Pradhan et al. (2010) reported that supercritical fluid CO2-extracted flaxseed oil is superior in terms of yield of ω-6-fatty acid and ω-3-fatty acid as compared to soxhlet and screw press techniques. The composition of oil extracted from SFE was almost same as compared to screw press. However, the yield of fatty oil was comparatively less (27.8%). Supercritical fluid CO2-extraction is an ecofriendly process and the extracted oil is of higher quality without any solvent residue. Hence, it can be used preferably in different food products. Khattab and Zeitoun (2013) compared the oil that was extracted from accelerated solvent extraction (ASE), super critical fluid extraction (SFE), and solvent extraction (SE). It was reported that oil yield was high in case of SE and ASE as compared to SFE. However, supercritical fluid CO2-extracted oil has higher SDG contents, phenolics and PUFAs.

3.2 Ultrasonic assisted extraction (UAE)

Zhang et al. (2008) reported the use of ultrasonic assisted extraction of flaxseed oil. The reports suggested that by using UAE there was increase in the oil yield along with low solvent consumption and significant decrease in the extraction time. Long et al. (2011) used ultrasound extraction assisted with an aqueous enzymatic process (AEP-UE) for the extraction of flaxseed oil. About 68.1% of oil was extracted with the help of this technique. The oil extracted by AEP-UE has 1.5% more unsaturated fatty acids as compared to organic solvent extraction technique. This technique can be potentially applied in oil industry as it is an ecofriendly technology as compared to solvent extraction technique.

3.3 Three-phase partitioning (TPP)

Tan et al. (2016) reported that enzyme-assisted three-phase partitioning method can be used for the extraction of flaxseed oil. The entire procedure was divided into two parts: firstly the enzymolyisis in which flaxseed was hydrolysed with enzymes and secondly, three-phase partitioning (TPP) for extraction of flaxseed oil. The results suggested that this technique gives higher yield of oil and quality as compared to other techniques. It has potential to be utilized for large or pilot scale production of flaxseed oil.

3.4 Subcritical extraction

Subcritical extraction is based on the technique of using a solvent as an extractant. Piva et al. (2018) used subcritical propane for the extraction of flaxseed oil. Propane is preffered as solvent as it has high solvation properties and is non-toxic. The use of propane led to yeild enchancement and the quality of the brown flaxseed oil was better as compared to mechanical extraction. The oil extracted by this technique showed that the free fatty acid content is less as compared to commercial oil (0.95% wt vs 1.37% wt).

4 Different techniques for extraction of lignans and phenolic acids

A number of studies have been undertaken on the antioxidant potential of flaxseed and on its phenolic compounds. Different phenolics present in flaxseed are lignans, phenolic acids, flavonoids, Phenylpropanoid glucoside and tannins. Flaxseed (Linum usitatissimum) is the major natural source of lignan with secoisolariciresinol diglucoside being the main lignan compound. Lignans are configured by the joining of two cinnamic acid residues and a 2,3-dibenzylbutane nucleus (Cacace and Mazza, 2006). The phenolic acids which are present in large concentration in flaxseed are ferulic acid and p-coumaric acid. There is rise in the intrest on SDG because of its different potential health benefits like anticarcinigenic, estrogenic and other antioxidant properties (Zhang et al., 2007). Thus, there is a need for development of novel approach for efficient extrication of these compounds. Table 2 put on the view of different techniques used for the extraction of phenols.

Table 2

Different techniques for the extraction of lignans and phenolic acids.

4.1 Microwave-assisted technique (MAE)

Beejmohun et al. (2007) for the first time put on an application of microwave assisted technique for the extraction of SDS, p-coumaric acid and ferulic acid. The comparison was also carried out with the conventional extraction techniques used for the extraction of these compounds. The SDS, p-coumaric acid and ferulic acid content found was 16.1 mg/g, 3.7 mg/g, 4.1 mg/g on subjecting the sample to three-minute MAE. Microwave-assisted extraction produced greater amount of phenolic compounds in comparison with other methods in short duration of time. Zhang et al. (2007) executed microwave-assisted extraction of secoisolariciresinol diglucoside (SDG) from flaxseed hull. The MAE extraction technique was also compared to stirring extraction and soxhlet extraction techniques. The comparison of three techniques brought out to a conclusion that the yield of SDG by microwave-assisted extraction was greater (11.7 g SECO kg−1 DFH) as compared to stirring technique (10.0 g SECO kg−1 DFH), however, less than that of soxhlet extraction (7.60 g SECO kg−1 DFH).

4.2 High voltage electric diffusion (HVED)

Boussetta et al. (2013) described the effect of high voltage electric, mild temperature treatment and concentration of ethanol on the yield of lignans and polyphenols from flaxseed cake. HVED helps in the rupturing of plant tissues and thereby releasing the intracellular compounds. The results suggested that the presence of ethanol shows a synergic effect in the extrication of polyphenols in both crushed and uncrushed flaxseed cake. The diffusion of polyphenols from HVED treated sample was found to be 2.6–2.7 × 10−9 m2/s (without ethanol) and 3.0–4.2 × 10−9 m2/s (with ethanol). The yield of lignan was found to be comparitively less than that found in literature. This technique is not as effective as alkaline or acid hydrolysis but it can be presumed that a combination of these techniques could create a new opportunity to reduce the application of chemicals and accelerate the solid–liquid separation step.

4.3 High-performance liquid chromatography

Eliasson et al. (2003) studied the high performance liquid chromatographic analysis (HPLC) of hydroxycinnamic acid glucosides and secoisolariciresinol diglucoside (SDG) in milled defatted flaxseed flour. The extraction was brought about by two methods; one was with dioxane-ethanol succeeded by the alkaline hydrolysis and other was extraction by direct alkaline hydrolysis. The results exhibit that yield was higher in the case of direct alkaline hydrolysis. The hydroxycinnamic acid glucosides and SDG were determined. Results suggested that a lot of variation was found in the phenolic glycosides and SDG content in Swedish flaxseed samples. Johnsson et al. (2000) carried out high performance liquid chromatography for the analysis secoisolariciresinol diglucoside. The analytical method included the steps of extraction, base hydrolysis, solid-phase extraction, and high performance liquid chromatography. Studies on the changes in SDG content were carried out in 15 cultivars of Sweden and Denmark flaxseed samples. The results suggested that the content varied from 6.1 and 13.3 mg/g (whole flaxseeds) and 11.7 and 24.1 mg/g in (defatted flaxseed flour).

4.4 Pressurized low polarity water extraction

Pressurized low polarity water extraction was used for extraction of lignans and bioactive compounds from the whole flaxseed. The effect of different process conditions like optimum flow rate, temperature, and total volume were studied to extract the selected polyphenols. The results concluded that the maximum amount of lignan and bioactive compounds are produced at 160 °C and 5.2 MPa. However, on dry basis, it takes place at 140 °C and 5.2 MPa. The flow rate of 0.5 mL/min is suitable for extraction of secoisolariciresinol diglucoside (SDG) and flow volume of 30–40 mL/g is needed for maximum retrieval. Bed depth to ID ratios of 5–18 led to maximum yield that is 90–95%. This technique is reported to be superior to other techniques because it is cost effective, less time consuming, higher recovery, less use of energy and raw material (Cacace and Mazza, 2006).

4.5 Enzyme-assisted extraction

Puri et al. (2012) stated that enzymes enhances the production of bioactive compounds from the plant source. The enzymes such as cellulases, pectinases and hemicellulases are competent of rupturing the plant cell wall which result in more yeild of the bioactive compounds from the plants. The enzymes increase the cell wall permeability by hydrolyzing the cell wall component and result in high extraction. Renouard et al. (2010) brought about the production of secoisolariciresinol from whole flaxseed and flaxseed hull. Enzymes are very productive for the recovery of variety of compounds from the flaxseed. The enzymes used were cellulase and b-glucosidase. Cellulase enzyme R10 from Trichoderma reesei proved out to be better than b-glucosidase enzyme. Under optimum conditions, the maximum yield of secoisolariciresinol was found out to be 40.75 mg g−1 in hulls of cv. Baladin and in whole seeds of cv. Barbara, it was found out to be 15.20 mg g−1. The recovery was higher as compared to the papers which are published previously. This is an efficient method to recover valuable yield of SECO for in vitro and vivo experiments.

4.6 Ionic liquid-based ultrasonic assisted extraction

Tan et al. (2015) carried out ionic liquid-based ultrasonic assisted extraction of SDG which was futher purified by onic liquid-based aqueous two-phase system (IL-ATPS). These techniques are suggested to be effective for extraction of SDG and bioactive components of plants. The efficiency of extraction was found out to be 93.35% under optimum conditions of pH(11), temperature(22 °C), IL(45.86%) and Na2SO4 (8.27%).

5 Different techniques for extraction of proteins

The flaxseeds are an efficient source of proteins. The proteins are of prime importance as they help in the reduction of hypertension and heart diseases because the plant proteins tend to reduce the cholesterol levels. The type of extraction process affects the quality of protein. There are different techniques which can be used to extract the protein from plant source. Table 3 shows the various techniques for the extraction of proteins from the flaxseed.

Table 3

Different techniques for the extraction of proteins.

5.1 Pressurized low polarity water technique

Ho et al. (2006) used pressurized low polarity water technique for the extraction of proteins, lignans and carbohydrates from the defatted flaxseed meal. Several conditions like temperature, pH, solvent to solid ratio and introduction of co-packaging material for optimal recovery of bioactive compounds were also analyzed. The ideal conditions required for the production of proteins were 160 °C, pH 9 and solvent to solid ratio is 210 mL/g meal. The temperature recommended for the isolation of carbohydrates and protein was 130–160 °C as they are highly potent to thermal decomposition. Pressurized low polarity water technique has an ability to be a commercially effective technique for extraction of proteins.

5.2 Enzyme-assisted extraction

Tirgar et al. (2016) investigated the effect of extraction method on the flaxseed protein concentrates (from cold pressed flaxseed meal). The extraction techniques used were an enzyme-assisted, alkali isoelectric precipitation and enzyme solvent assisted. The comparison was also carried out with pea protein concentrates. The yields of proteins by enzyme-assisted solvent extraction (86.80%) were compared to enzyme-assisted (65.08 %) and alkali isoelectric precipitation (51.5 %). The results also suggested that though the protein yield was less in alkali-extracted flaxseed protein concentrate but it had highest emulsifying properties as compared to other two extraction methods. The study recommended the use of alkali- and enzymatic-extraction techniques for the emulsion based foods that require good emulsifying properties. Enzyme-assisted solvent extraction can be used to produce protein for a food system. Thus, these extraction techniques can be used to produce proteins with specific functional properties.

6 Conclusion

Different types of extraction methods have been used to extract oil, lignans, phenolic acids and proteins. Traditional methods like mechanical pressing and soxhlet have many limitations. Hence, there is a need to explore new techniques for the extraction of different biologically active components. Among the newer techniques, enzymes-assisted three-phase partitioning and ultrasound extractions are giving more yield and are fast processes. Pressurized low polarity extraction and microwave-assisted extraction are giving more yield of lignans and phenolic acids. For the extraction of proteins, enzymatic extraction is giving better yield and having less limitations as compared to other methods. Cost savings, environmental and safety concerns, and nutrition issues seem to be achievable by the successful development of alternative techniques for conventional oil extraction techniques.

Conflict of interest

Authors declare that they do not have any conflict of interest.

Acknowledgements

We would like to acknowledge Lovely Professional University for providing equipments and their support for carrying out the research work.

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Cite this article as: Sharma M, Dadhwal K, Gat Y, Kumar V, Panghal A, Prasad R, Kaur S, Gat P. 2019. A review on newer techniques in extraction of oleaginous flaxseed constituents. OCL 26: 14.

All Tables

Table 1

Different techniques for the extraction of oil from flaxseed.

In the text
Table 2

Different techniques for the extraction of lignans and phenolic acids.

In the text
Table 3

Different techniques for the extraction of proteins.

In the text

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