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Table 3
Analysis of endogenous lipase activity.
| Type | Method of assaying lipase | Substrate | Temperature | pH | Reference |
|---|---|---|---|---|---|
| in vitro | Powder obtained from maceration of mesocarp tissue with liquid nitrogen | Glycerol [14C] trioleate | 20–45 °C | – | (Henderson and Osborne, 1991) |
| Oleic acid released from emulsion | Glycerol [14C] trioleate | Maximum at 18°C | – | (Sambanthamurthi et al., 1995) | |
| Chemical extraction | Olive oil | Optimum temperature: 45°C | Optimum pH: 9–10 | (Ebongue et al., 2006) | |
| Active at 20–50 °C | Not active below 7.0 and above 11.5 | ||||
| in vivo | Cold induction assay | – | Maximum at 5°C | – | (Sambanthamurthi et al., 1991) |
| Powder obtained from maceration of mesocarp tissue with liquid nitrogen | Glycerol [14C] trioleate | 20–45 °C | – | (Henderson and Osborne, 1991) | |
| Cold induction assay | – | Maximum at 5°C | – | (Cadena et al., 2013) | |
| No FFA detected at room temperature or higher (45°C) |
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