Analysis of endogenous lipase activity.
|Type||Method of assaying lipase||Substrate||Temperature||pH||Reference|
|in vitro||Powder obtained from maceration of mesocarp tissue with liquid nitrogen||Glycerol [14C] trioleate||20–45 °C||–||(Henderson and Osborne, 1991)|
|Oleic acid released from emulsion||Glycerol [14C] trioleate||Maximum at 18°C||–||(Sambanthamurthi et al., 1995)|
|Chemical extraction||Olive oil||Optimum temperature: 45°C||Optimum pH: 9–10||(Ebongue et al., 2006)|
|Active at 20–50 °C||Not active below 7.0 and above 11.5|
|in vivo||Cold induction assay||–||Maximum at 5°C||–||(Sambanthamurthi et al., 1991)|
|Powder obtained from maceration of mesocarp tissue with liquid nitrogen||Glycerol [14C] trioleate||20–45 °C||–||(Henderson and Osborne, 1991)|
|Cold induction assay||–||Maximum at 5°C||–||(Cadena et al., 2013)|
|No FFA detected at room temperature or higher (45°C)|
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