Open Access
Issue
OCL
Volume 27, 2020
Article Number 6
Number of page(s) 8
Section Quality - Food safety
DOI https://doi.org/10.1051/ocl/2020002
Published online 11 February 2020

© E. Ghanbari Shendi et al., Hosted by EDP Sciences, 2020

Licence Creative Commons
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

1 Introduction

Extra virgin olive oil (EVOO) is virgin olive oil which has a free acidity, expressed as oleic acid, of not more than 0.8 g per 100 g, and the other characteristics of which correspond to those fixed for this category in this standard (IOC Regulation, 2013). Extra virgin olive oil (EVOO) is extracted from the olive fruit by using physical methods and it is ready to consume after extraction without refining. So, it conserves many natural bioactive compounds such as vitamins, phenolics, and sterols as well as a good balance of fatty acids. EVOO has a unique aroma, taste, color and nutritive features which identify it from other edible vegetable oils. The chemical composition of olive oil varies depending on the genetic, geographic, agronomic and process and storage (Boskou, 2006). Shelf life of virgin olive oil is longer than other edible oils, because of the presence of antioxidants such as biophenols and α-tocopherol. Major and minor compounds such as free fatty acids, unsaturated hydrocarbons, enzymes, pigments and trace of metals were affected negatively due to storage time and storage conditions. Storage of olive oil under nitrogen pressure in a dark place at room temperature (25–30 °C or lower) increases shelf life (Boskou, 2006). Conflicting results were reported on the effect of storage on the unsaturated fatty acid composition in the literature. A decrease in chlorophyll and carotenoid contents and an increase of oleic acid percentage were also reported ( Morelló et al., 2004). This increase of oleic acid percentage has been found in the fatty acid composition as a result of the degradation of polyunsaturated acids (linoleic acid and linolenic acid). Vitamin E (α-tocopherol) loss reached up to 79% in four months, whereas < 45% of the phenols were lost under diffused light during storage (Okogeri and Tasioula-Margari, 2002). A positive correlation was found between the age of the oils and the tyrosol to total phenols ratio (Cinquanta et al., 1997). EVOO with high antioxidant contents was still “excellent” after 240 days of storage at 40 °C (Lavelli et al., 2006). Although an important loss of total phenol content was seen in commercial Arbequina virgin olive oil after 12 months of storage, no changes in some phenolics (tyrosol and hydroxytyrosol) and aromatic hydrocarbon contents of freezed samples were reported up to 12 months (Mulinacci et al., 2013). Psomiadou et al. (2000) suggested a good handling is quite important to keep high α-tocopherol levels of Greek VOO under domestic conditions for two years.

Since Turkey is the motherland of olive trees, there are many minor olive varieties, produced and consumed in the local market. When the growers of these minor varieties have problems in processing or trading, they can leave these olive groves for something else. We wondered for these olive germplasms because they could be disappeared. Most of the minor olive varieties, like Tavşan Yüreği, have been processed for olive oil (and/or table olive) for centuries in their locations, but never in high-quality EVOO. So nobody has been known the real taste and the amount of minor compounds like polyphenol, contents of these olive oils. For that reason, we had prepared a project for design a mobile olive oil extraction unit in order to produce high quality olive oil from Tavşan Yüreği and other, not-well-known, minor varieties, in its origin. Tavşan Yüreği olive is originated and located mainly Döşemealtı and Kepez villages of Antalya province, located in Western Mediterranean Region. The number of olive trees of Tavşan Yüreği cv. is about 250 000. Most of the trees are older than 90 years. Total yield is about 10 000 tons per year. It is used mainly for green table olive and the rest processed for olive oil but for local consumption. While early harvesting fruit has 10% olive oil, at the end of ripening it increased up to 20% level. In this study, we aimed to perform the best processing method for olive oil production from Tavşan Yüreği olive cultivar grown locally in Antalya. For this purpose, a mobile olive oil processing unit was designed and used for cold press olive oil production. Chemical composition and sensory evaluation were monitored during 12 months’ storage. The quality of Tavşan Yüreği oil was approved by the quality prize “Gold Award 2015” in Monocultivar Olive Oil Expo2015 in Milan and The New York International Olive Oil Competition.

2 Materials and methods

2.1 Production of extra virgin olive oil (EVOO)

Mobile Olive Oil Processing Unit (MOOPU) with state-of-the-art Oliomio equipment was designed in order to produce high-quality EVOO (Fig. 1). A special container was constructed and equipped with a knife crusher and a two-phase horizontal decanter (Oliomio D500, Italy). The MOOPU is a unique olive extraction unit (with 600 kg/h capacity industrial scale but laboratory conditions in terms of hygiene and quality) which was used for oil extraction. It is extremely important because there is no olive oil factory where the olives were processed at optimum conditions in the origin provinces of the local varieties. The mobile unit is an articulated lorry with a special semi-trailer measuring 2438 × 12 192 × 2896 mm which is divided into three separate sections. The first section is the olive accepting unit including; bunker, leaf removers, washer and crusher units of the system. The second section is a processing unit including malaxer, decanter, filter, and bag-in-box filling machine. The third section is support unit placed a power plant and a water supply tank. Processing unit was equipped with an air conditioner, isolation and filter ventilation systems and protected for temperature changes, dust and odor. MOOPU carried by a trailer truck to orchards in the 2015–2016 season. Olive fruits (about one metric ton) were harvested by hand picking in the early harvest period and processed to “cold press” VOO in the MOOPU in one batch. The process was completed in a couple of hours. The olive paste was prepared after crushing by a hammer mill and the paste was mixed in the malaxer at 27 °C for 15 min (Cold press). After decantation EVOO (about 100 liters) was filtered and filled in 250 ml amber glass bottles (headspace: 4 cm) by nitrogen gas. The bottles (24 bottles, 12 of them for chemical and 12 of them for sensorial analysis) were stored at room temperature (18–24 °C) up to 12 months.

thumbnail Fig. 1

Mobile Olive Mill (MOM) design. Section 1: olive accepting unit including; bunker, leaf removers, washer (1) and crusher (2); section 2: processing unit including malaxer (3), decanter (6), filter (9) and filling machine (10); section 3: support unit placed a power plant and a water supply tank.

2.2 Chemical analysis

Free fatty acid content and peroxide value were performed according to the EC 2598/91 (European Commission Regulation, 2013) and AOCS Cd 8-53 methods (2003), respectively. Analytical grade chemical materials were used for free fatty acid content and peroxide value assays (ethanol, diethyl ether, acetic acid, chloroform). Color values (L, a, b values) were determined by spectrophotometry (Minolta, CM-3600d, Japan). UV absorbance was performed according to the IOC method COI/T.20/Doc. No. 19/Rev. 3. The UV values were measured at 232, 266, 270 and 274 nm by using UV Spectrophotometer (Agilent 8453, USA). For this propose, 0.25 g olive sample was weighed in to 50 ml falcon tube and 25 ml cyclohexane was added on it. Falcon tube was shaken for 1 min and 1 ml of this solution was filled in a quartz cuvette. ΔK values were calculated with the following formula:

ΔK = K270 – [(K266 + K274) / 2].

In the present study, chemical and sensory assays were performed monthly, but chromatographic analysis including total phenolic content, phenolic composition and tocopherol content were measured quarterly or every six months due to the slight changes on the minor compounds and limited project budget.

2.3 Total phenolic content

Polar fraction was extracted and used for total phenolic and phenolic composition analysis. Polar fraction was obtained according to the methods of Romani et al. (2007) and Inarejos-García et al. (2009). 2.5 g of EVOO sample was weighed into a falcon tube. Hexane (6 ml) was added and shaken for 1 min. This solution was passed through solid phase extraction (SPE) cartridge (Superclean LC-Diol, USA) and collected in a glass tube. Then hexane (6 ml) and 4 ml hexane: ethyl acetate (85:15, v/v) were passed through the SPE cartridge, respectively. The cartridge was washed with methanol: deionized water solution (1:1, v/v) and phenolic extract was evaporated (UniEquip Univapo 100 ECH, Canada). After addition of 2 ml methanol: deionized water solution (1:1, v/v) the tubes vortexed for 30 s. For determination of total phenols, Folin & Ciocalteu method was used and the results were expressed in terms of gallic acid equivalent (mg gallic acid/kg oil) (Romani et al., 2007; Inarejos-García et al., 2009). Ultra high-performance liquid chromatography (UHPLC, Thermo Scientific Dionex Ultimate 3000, USA) and C18 column (4.6 mm inner diameter × 250 mm length and 5 mm particle diameter; Thermo scientific acclaim 120) was used for determination of phenolic profile. Prepared phenolic extract (1 ml) was filtered through 0.45 μm. Syringe filter (Merck, PVDF, Millipore Millex-HV, Germany) and poured into an amber vial. Column temperature was fixed at 30 °C and acetic acid: deionized water (1:1, v/v) (A), methanol (B), acetonitrile (C) were used in a gradient flow program as the mobile phase. In the gradient program eluents were 2.5%B, 2.5%C, and 95%A solution up to 60 min. The flow rate was 1 ml/min and diode array detector (DAD) detector was set in 280, 320 and 335 nm. HPLC grade hexane, methanol, acetonitrile and ethyl acetate were used in total phenolic content and phenolic compounds assays. Apigenin, cafeic acid, gallic acid, luteolin, m-coumaric acid, p-coumaric acid, oleuropein, syringic acid, trans-ferulic acid, vanilic acid, vanillin, tyrosol, 3-hydroxy tyrosol, 3,4-dihydroxy benzoic acid, 4-hydroxy benzoic acid, 4-hydroxy phenyl acetic acid were used as standards.

For tocopherol composition, EVOO sample (2 g) was weighed into a 25 ml volumetric flask (AOCS Official Method Ce 8-89, 1997). A quantity of hexane (HPLC grade) was added and shaked to dissolve the sample. Flask was made up to volume with the same solvent. The solution was passed from syringe filter (0.45 µm) (PVDF, Millipore Millex-HV) in to the HPLC vial. The samples (20 μl) were injected into an HPLC (UHPLC: Ultra-High Performance Liquid Chromatography (Dionex Ultimate 3000) equipped with. LiChrosorb SI 60-5 column (4.6 mm I.D × 250 mm length and 5 µm particle size). Column temperature was fixed at 30 °C during the process. Flow rate of analysis was 1 ml/min. For mobile phase, isopropanol: hexane (0.5:99.5, v/v) isocratic mix was used and chromatograms were collected at 292 nm wavelength. Analysis time was 30 min and the injection volume was 100 μl. Amounts of α, β, ɣ and Δ tocopherols were determined by using tocopherol standards.

2.4 Sensory analysis

Every month EVOO samples were evaluated by the Ayvalık Olive Oil Tasting Laboratory accredited by International Olive Council (IOC Regulation, 2015) and TURKAK (Turkish Accreditation Agency) according to the method for the organoleptic assessment of virgin olive oil (COI/T.20/Doc. No. 15/Rev. 8, November 2015). Eight trained tasting panels were able to assess the oils to determine the levels of positive attributes, such as fruitiness, bitterness and pungency. Negative attributes arising due to poor quality fruit, incorrect processing or storing, such as rancidity, musty and fusty, were determined by sensory panel. Descriptors were evaluated on a 0–10 intensity scale (a number between 0 and 10).

2.5 Statistical analysis

Statistical analysis was performed by SPSS 17 (SPSS Inc.Chicago, IL) statistical software and using One-way ANOVA method. All analysis was performed at least duplicate for each month EVOO samples and differences among all groups were determined by Duncan test. The confidence level for statistical analysis was 99%.

3 Results

3.1 Chemical analysis

Free fatty acidity, peroxide value and UV absorbance values of the olive oils extracted by Mobile Olive Oil Processing Unit (MOOPU) were shown in Table 1. Although a slight increase was observed during storage all samples could be classified as extra virgin olive oils according to the International Olive Oil Council (IOC) standards (Tab. 1). Free fatty acidy which is an important criterion for the classification of olive oils increased slightly in the third month and no change was observed until the end of storage time. It is well known that free acidity increased with storage depending on the packaging material, storage conditions and time (Lavelli et al., 2006; Clodoveo et al., 2007; Méndez and Falqué, 2007; Abdalla et al., 2014; Baiano et al., 2014).

Peroxide value (PV), which is the primary oxidation indicator, showed an increasing trend up to the end of the storage period (Tab. 1). The minimum level of PV was observed at the beginning of the storage. Significant increases were reported on the PV of olive oil samples from different cultivars during short-term (30 days) and long-term (6 years) storage in different packaging materials at different conditions (Okogeri and Tasioula-Margari, 2002; Lavelli et al., 2006; Clodoveo et al., 2007; Abdalla et al., 2014).

Absorption values (K232 and K270) are one of the quality parameters and helps to differentiate between extra virgin, virgin and regular olive oil as defined by the International Olive Council (IOC). UV absorbance values (K232 and K270) which are indicator of oxidation changed during storage significantly. A low absorption value is a sign of a high-quality extra virgin olive oil, whereas low-quality oil shows a greater level of absorptions in the UV region. UV absorbance values (K232 and K270) which are advanced oxidation products changed during storage significantly (P < 0.01). K232 values decreased up to the third month. The sharp increase was observed in the fourth, sixth and seventh month and it was stable near the end of storage time. The highest and the lowest K270 values were in the fifth and first months, respectively (Tab. 1). Except for second, third, fifth and seventh months of storage period, K270 values were under IOC (International Olive Council standards) limitations. ΔK values were zero or below zero (results are not shown). These results are in agreement with the related literature (Okogeri and Tasioula-Margari, 2002; Caponio et al., 2005; Del Caro et al., 2006; Lavelli et al., 2006; Gómez-Alonso et al., 2007; Méndez and Falqué, 2007; Baiano et al., 2014). Baiano et al. (2014) reported that K232 value of Coratina olive oil increased up to the sixth year, then it decreased and the end of storage an increase was observed. Gutiérrez and Fernández (2002) showed that K270 and sensory evaluation indices of Picual and Hojiblanca olive oils decreased during storage at 2 °C in darkness and 30 °C in illumination.

Table 1

Oxidative stability parameters and color values of Tavşan Yüreği (Antalya) olive oils during 12 months of storage.

3.2 Color analysis

Although the color is not regarded as an important quality feature for olive oil, it has a great influence on consumer acceptance. The color of virgin olive oils is depended on olive maturity and process conditions. Color values (L, a and b values) were measured during storage. The maximum for L is 100, which would be a perfect reflecting diffuser. The minimum measuring for L would be zero, which would be black. The a and b have no specific numerical limits. Positive a is red and negative a is green. Positive b is yellow and negative b is blue. Analysis of color (L, a and b values) showed that the color of olive oil samples changed significantly during storage (Tab. 1). It has been attributed to decomposition of color pigments such as chlorophylls, pheophytins, xanthophylls and carotenes (Boskou, 2006). The lowest L value (lightness) was seen in the eighth month. The L values was increased to 34.15 after 9 months and then decreased lightly. Fluctuations were observed in a (redness) and b (yellowness) values during storage. The highest b value was obtained for the tenth month. After this month there was a decreasing trend in b value. The highest a value also observed in ninth and twelfth months.

3.3 Tocopherol profile

There are four natural tocopherols α, β, γ, δ-forms are available in olive oil. The α-tocopherol (vitamin E) is the major antioxidant (150 and 300 ppm) present in olive oil and it displays antioxidant properties and important role in the olive oil stability (Cert et al., 2000). Tocopherol (α, β, ɣ) profiles of the EVOO samples were determined every three months during storage (Tab. 2). The results showed that total tocopherol content decreased from 380.16 to 294.61 ppm with increasing storage time. The lowest tocopherols content was obtained at the end of storage. It means that 22.38% of α-tocopherol, 65.59% of β-tocopherol and 34.48% of ɣ-tocopherol contents were decomposed during storage. Decreasing in α-tocopherol was lower than other tocopherol isomers. Although a rise of α-tocopherol content was observed for the Arbequina oils, decreases in this content between 47% and 70% for Picual and Hojiblanca oils were reported respectively, at 60 °C. (Escudero et al., 2016).

Table 2

Tocopherol Content of Tavşan Yüreği (Antalya) olive oil monocultivar during 12 months’ storage (ppm).

3.4 Total polyphenolic compounds

Phenolics are important minor components in olive oil which, the powerful antioxidant effect, contribute to shelf life stability and sensory properties of olive oil. Total polyphenol contents of the samples were presented in Table 3. The highest total polyphenol values were determined at fresh oil which decreased with time. But the decreases were not dramatic as well as tocopherols, after one year 15% of total polyphenols were decomposed in EVOO samples. Detrimental effects of factors such as oxygen, light, heat and time on phenol compounds of olive oil have been reported in a number of studies. After a short term or long term storage, significant decreases in total polyphenols were reported for monocultivar and commercial olive oils (Morelló et al., 2004; Clodoveo et al., 2007; Baiano et al., 2009; Abdalla et al., 2014, Baiano et al., 2014). The loss of phenolics among the varieties varied and this differences have been attributed to a result of initial differences in oil acidity (Brenes et al., 2001). In Turkey, phenolic contents of local monocultivar (Halhali, Egriburun, Hasebi, Karamani and Saurani) olive oils and commercial monocultivar (Gemlik, Memecik, Ayvalık, Uslu and Domat cv.) olive oils ranged 64–321 mg caffeic acid/kg and 23.69–153.64 caffeic acid/kg respectively (Kara et al., 2018).

Table 3

Changes in Total phenols of Tavşan Yüreği (Antalya) EVOOs during 12 months of storage (ppm).

3.5 Phenolic profiles

Phenolic profiles of the samples were determined every six months (Tab. 4). 3,4-dihydroxy benzoic acid, tyrosol, 4-hydroxy benzoic acid, syringic acid, m-coumaric acid, luteolin, and apigenin were identified and quantified in the polar fraction of olive oils. Luteolin was the most abundant phenolic that has been identified for all samples. Except tyrosol, other phenolic compounds content decreased with storage. These results confirmed that filtration caused significant change in the phenolic profile. The main reason of this effect could attributed to the filtration process. Filtration process can decrease water-soluble phenolic content and antioxidant in VOO (Ngai and Wang, 2015). Yorulmaz (2009) reported that luteolin was the most abundant phenolic compound following trans-cinnamic acid and luteolin-7-glucoside. They also quantified tyrosol, syringic acid, p-coumaric acid, luteolin-7-glucoside, trans-cinnamic acid, luteolin and apigenin in Turkish olive oils extracted from different olive varieties. Montedoro et al. (1992) reported that dialdehydic form of elenolic acid linked to 3,4-dihydroxyphenyl ethanol (3,4-DHPEA), p-hydroxyphenyl ethanol (p-HPEA), vanilic acid, cafeic acid, 3,4-DHPEA-EDA, 3,4-DHPEA-EA had been identified in olive oils. Morelló et al. (2004) suggested that although storage did not appear to have any effect on vanilic acid or vanillin, which were present at low concentration there was a significant decrease in the concentration of the rest of the quantified phenolic compounds. That reduction was more marked in the secoiridoid derivatives such as dialdehydic form of elenolic acid linked to hydroxytyrosol (3,4-DHPEA-EDA), p-HPEA-EDA (dialdehydic form of elenolic acid linked to tyrosol) and 3,4-DHPEA-EA (oleuropein

Aglycon) indicating a more active participation in the oxidative processes as they were more easily oxidized. Among the most representative phenolic compounds in olive oil, lignans seem to be the most stable during oil storage. García et al. (2003), Gómez-Alonso et al. (2007) and Mulinacci et al. (2013) showed an increase tyrosol and hydroxytyrosol contents over time due to hydrolytic processes of the secoiridoidic derivatives. Gómez-Alonso et al. (2007) stated that the main phenols were oleuropein aglycon, dialdehydic form of elenolic acid linked to tyrosol (p-HPEA-EDA) and hydroxytyrosol (3,4-DHPEA-EDA). Baiano et al. (2014) reported that there were increasing and decreasing trends in phenolic compounds (3,4-DHPEA, p-HPEA, vanillin, p-coumaric acid, 4-(acetoxyethyl)-1,2-dihydrobenzene (3,4-DHPEA-AC), 3,4-DHPEA-EDA, p-HPEA-AC, p-HPEA-EDA, 1-acetoxipinoresinol + trans-cinnamic acid, p-HPEA-EA) content.

Table 4

Changes in phenolic compounds of Tavşan Yüreği (Antalya) olive oils during 12 months of storage (ppm).

3.6 Sensory analysis

Tavşan Yüreği extra virgin olive oil had a green fruity aroma characterized by caramel, honey, blossom, green apple, grass, freshly cut grass, almond and spring notes. It had no-defect and fruitness is higher than bitterness even pungency (Fig. 2). Fruitness decreased during a year storage time. It was received from 4 to 3.5 at the end of the year. So it has medium fruitiness according to IOC Regulation (3.0 < Me ≤ 6.0). During storage of samples, bitterness has been reduced from 4.5 to 3.1. The bitterness of EVOO samples changed in medium level (3.0 < Me ≤ 6.0). However, pungency was 4 out of 10 in the beginning and reduced from 5.5 to 3.5 in EVOO samples. Pungency also altered in the medium range (3.0 < Me ≤ 6.0). Tasting panel did not detect any defects in the samples during whole a year storage time in room temperature. This can be attributed to the high amount of polyphenols.

There are some compounds reported by different researchers to be the major components responsible for sensorial perceptions such as bitterness and pungency. Phenolic compounds, particularly secoiridoids generally decrease during EVOO storage. Because of the hydrolysis of secoiridoid derivatives in the hydroxytyrosol, tyrosol and elenolic acid and formation of oxidized phenols. By these reactions, bitterness and pungency which are positive attributes that are characteristic of a fresh EVOO, are decreasing (Bendini et al., 2009).

thumbnail Fig. 2

Sensory values of Tavşan Yüreği (Antalya) olive oils during 12 months of storage.

4 Conclusion

Tavşan Yüreği olive is harvested in small quantities from old trees in southwestern Anatolia especially Muğla and Antalya regions. Most of the minor olive varieties, like Tavşan Yüreği, have been processed for olive oil for centuries in their territories and these oils are not prominent due to their little recognition. Because generally small and/or damaged fruits that are not suitable for table olive production are used for olive oil production. To the best of our knowledge, this is the first work addressing EVOO extracted from Tavşan Yüreği cultivar in a pilot scale in terms of chemical and sensory properties.

The results obtained in this study showed that Tavşan Yüreği olive oils processed at optimum conditions have a unique sensory and chemical composition with a good oxidative stability due to high tocopherols and phenolics contents that are uncommon in most of the commercial olive oils in Turkey. Indeed, fresh EVOO had a sensory profile with the levels of intense fruitiness and medium bitterness and pungency with an equilibrated taste. Although fruitiness score decreased from intense to medium level other positive attributes (bitterness and pungency) were still medium level after one year. These results are of great importance to the olive oil industry for the production of high-quality monovarietal virgin olive oil from varieties with peculiar and differential characteristics and will provide some support to preserve Anatolia olive resources. According to the result of this study, mobile olive oil processing unit could be used for other local varieties in each origin province.

Abbreviation

EVOO: Extra virgin olive oil

VOO: Virgin olive oil

IOC: International Olive Council

MOOPU: Mobile Olive Oil Processing Unit

Acknowledgment

This study was performed as a part of a project entitled “Improvement of Possibilities of Using Olive Genetic Resources in their Own Ecology by Conservation of Their Species Characteristics” The authors are grateful for the financial supports that were provided by the Republic of Turkey, Ministry of Science, Industry and Technology for financial supports (Project Number: SANTEZ-0560-STZ-2013-2).

References

Cite this article as: Ghanbari Shendi E, Sivri Ozay D, Ozkaya MT, Ustunel NF. 2020. Determination of chemical parameters and storage stability of extra virgin olive oil extracted by Mobile Olive Oil Processing Unit. OCL 27: 6.

All Tables

Table 1

Oxidative stability parameters and color values of Tavşan Yüreği (Antalya) olive oils during 12 months of storage.

Table 2

Tocopherol Content of Tavşan Yüreği (Antalya) olive oil monocultivar during 12 months’ storage (ppm).

Table 3

Changes in Total phenols of Tavşan Yüreği (Antalya) EVOOs during 12 months of storage (ppm).

Table 4

Changes in phenolic compounds of Tavşan Yüreği (Antalya) olive oils during 12 months of storage (ppm).

All Figures

thumbnail Fig. 1

Mobile Olive Mill (MOM) design. Section 1: olive accepting unit including; bunker, leaf removers, washer (1) and crusher (2); section 2: processing unit including malaxer (3), decanter (6), filter (9) and filling machine (10); section 3: support unit placed a power plant and a water supply tank.

In the text
thumbnail Fig. 2

Sensory values of Tavşan Yüreği (Antalya) olive oils during 12 months of storage.

In the text

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