Open Access

Table 1

Comparison of different gene silencing/mutagenesis technologies.

Mutagenic agent Effect on DNA structure Comments
or gene expression

Ethylmethane Point mutations, Large range of mutations including gain
sulphonate (EMS) CG to AT transitions and loss of gene function

Di-epoxy butane Point mutations, 6−8 bp High efficiency, hundreds of mutations
(DEB) deletions per genome
Difficult to locate mutations in genome

Gamma-rays or Chromosome breaks and Large insertions, deletions, and rearrangements
X-rays rearrangements Mostly loss of function mutations

Fast neutrons Deletions, < 1 kb Medium efficiency
Difficult to locate mutations in genome

T-DNA* DNA insertion Insertion of specific DNA sequences
Mostly loss of function mutations

Transposons* DNA insertion/deletion Low efficiency
Easy identification of mutations

RNAi or zinc-finger DNA insertion so that Targets single genes
constructs* transcribed gene product Causes loss of function
causes gene silencing Very effective
Requires knowledge of target gene function

Non-transgenic method


transgenic method

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