Volume 12, Number 4, Juillet-Août 2005
|Page(s)||320 - 325|
|Published online||15 July 2005|
Valorisation des huiles végétales d’origine béninoise par alcoolyse enzymatique
Ecole polytechnique d’Abomey-Calavi, Laboratoire de recherche en chimie et biologie appliquées (LARECBA), Unité de recherche en génie enzymatique et alimentaire, Université d’Abomey-Calavi, 01 BP 2009
2 Faculté des sciences et techniques, Département de biochimie et de biologie cellulaire, Université d’Abomey-Calavi, 01 BP 526 Cotonou, Bénin
3 Faculté des sciences et techniques, Laboratoire de chimie organique, Université d’Abomey-Calavi, 01 BP 526 Cotonou, Bénin
Accepté : 19 Mai 2005
Fatty acid esters synthesized from vegetable oils by alcoholysis reaction are potentially applied in cosmetic and as biodiesel fuel. The alcoholysis of cottonseed oil by lipases from various microorganisms was investigated. Among several microbial lipases tested in this work, some strains showed high hydrolytic activity, allowing activity for alcoholysis reaction. To select suitable alcohols and biocatalysts, alcoholysis screening in n-hexane and in a solvent free medium was developed. In organic solvent, only crude lipases from Pseudomonas strain were active in all alcohol tested. The best conversion (93%) was found with Pseudomonas fluorescens lipase (AK). In a solvent free medium, except Pseudomonas strain lipase, all crude microbial lipases were almost inactivated. To solve such lipase inhibition, a three step protocol addition of methanol was used. By using this enzymatic process, Pseudomonas fluorescens lipase immobilized on polypropylene support displayed high conversion (> 61%) in various beninese vegetable oils. Thus, giving added value to cheaper vegetable oils.
Key words: alcoholysis reaction / lipases / immobilization / natural vegetable oils / organic solvent / fatty acid esters
© John Libbey Eurotext 2005
Current usage metrics show cumulative count of Article Views (full-text article views including HTML views, PDF and ePub downloads, according to the available data) and Abstracts Views on Vision4Press platform.
Data correspond to usage on the plateform after 2015. The current usage metrics is available 48-96 hours after online publication and is updated daily on week days.
Initial download of the metrics may take a while.