Open Access

Figure 5.

image

Download original image

Effects of Spadin on neurogenesis, CREB activation and Serotoninergic neurotransmission. A) Spadin increases neurogenesis: (left), Representative photomicrographs of BrdU-labeled neurons in the dentate gyrus of the mouse hippocampus treated either with saline or with spadin (i.v., 10−6 M) for 4 days. (Right) Quantitation of BrdU positive cells of hippocampus treated with saline, fluoxetine or spadin (10−5 M) for 4 days. 85% of BrdU-labeled cells are positive to DCX. B) Enhanced spadin treatment-induced CREB activation in the hippocampus, as assessed by measuring phosphoCREB (pCREB) immunoreactivity. (left), Immunological distribution of pCREB in the mouse hippocampus after a 4-day i.v treatment. pCREB is phosphorylated in the cells near the subgranular zone (SGZ). Right: Quantification of pCREB positive cells/mm2 in hippocampal SGZ. C) Effect of spadin on the firing rate of DRN 5-HT neurons. Spadin (10−5 M in a 100 μL bolus) or its vehicle is i.p. administered. Recordings start 30 min after the injection, and are performed for a maximal duration of 210 min thereafter. Left: Samples of “descents” performed along the DRN, showing typical integrated firing rate histograms in a vehicle-, TREK-1 KO- or spadin-treated animal. Right: 5-HT neuron firing activity, calculated on the basis of all the cells recorded within the successive tracks performed along the DRN.

Current usage metrics show cumulative count of Article Views (full-text article views including HTML views, PDF and ePub downloads, according to the available data) and Abstracts Views on Vision4Press platform.

Data correspond to usage on the plateform after 2015. The current usage metrics is available 48-96 hours after online publication and is updated daily on week days.

Initial download of the metrics may take a while.