Plasmalogen deficiency is associated with major alterations of the brain cortex plasmalogen and fatty acid profiles that are not restored by AKG supplementation. A) Plasmalogen deficient (DAPAT^−/−) mice cortices were completely devoid of plasmalogens, while they represented 8% of total phospholipids in WT mice. 3 weeks of AKG-enriched nutritional supplementation through lactation was not able to increase plasmalogen levels in the brain cortex of plasmalogen deficient pups (KO + AKG). B) Plasmalogen profile of the brain cortex. The repartition of plasmalogen species was similar to that of the retina, but unlike to the retina no plasmalogens are detected in the cortices of supplemented pups. C) Fatty acid profile of the brain cortex. Plasmalogen deficiency was not associated with any significant change in saturated fatty acids (SFA), monounsaturated fatty acids (MUFAs), or polyunsaturated fatty acids (PUFAs) levels. AKG maternal supplementation does not affect any of the fatty acids concentrations in the pups cortices. * p <0.05; ** p <0.01; ***p <0.001. n = 2–8. One-way ANOVA with post-hoc Tukey’s multiple comparisons test. FAMEs: Fatty acid methyl esters; DMAs: Dimethylacetals.