Specific activity of pre-purified licuri lipase extract (PPLL) on various lipid substrates. (A) pH-dependent activities on olive oil, licuri oils tributyrin, phospholipids (Egg PC), and vinyl ester. Vinyl propionate was used at a concentration below its solubility in water (<86 mM; 100 μL/30 mL per assay) to measure the esterase activity of licuri lipase on a soluble substrate. (B) Interfacial activation: PPLL activity on vinyl propionate at substrate concentrations below (30 mM) and above (153 mM) the saturation (86 mM).