Genetic relationship and diversity among some Moroccan and introduced rapeseed (Brassica napus L.) varieties as revealed by molecular markers | OCL

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Table 2

List of 20 different ISSR primers and annealing temperatures used in the molecular characterization of 22 rapeseed varieties.

ISSR Primers	Type of repetition	Sequence (5’-3’)	Annealing temperature (°C)
F1 ^a,^b	Di	(CA)₆AT	43
F2 ^a,^b	Di	(CA)₆GC	58
F3 ^b	Di	(CA)6	43
F4 ^a,^b	Tri	(AGC)₄CT	58
F8 ^a,^b	Di	(AG)₈CC	56
F9^b	Di	(AG)₈CG	43
F11^a	Di	(CA)₈AC	56
F16 ^a,^b	Di	(GT)₈CG	58
IMA-5-Z^b	Di	(CA)₈GT	56
IMA9Z ^a,^b	Di	(GA)₈CG	56
IMA834z ^a	Di	(AG)₈CTT	50
IMA8Z	Di	(GA)₈GT	50
IMA834-2^b	Di	(AG)₈YT	50
UBC808-2^b	Di	(AG)₈C	50
UBC810^b	Di	(GA)₈T	50
UBC850^b	Di	(GT)₈TYC	50
UBC856^b	Tri	(GGAGA)3	56
IMA12-2 ^a,^b	Di	(CA)₈TC	56
ISS F1^b	Di	(AG)₈TA	50
ISSR1^b	Di	(AG)₈CA	56

Set of 8 primers allowing the distinction of the 22 genotypes.

17 primers to distinguish the three groups A, B and C in the dendrogram.

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