Open Access
Table 1
Parameters used for mass spectrometry analyses.
| Identification software | X!Tandem Piledriver (2015.04.01.1) http://www.thegpm.org/ |
| Filtering and inference software | X!TandemPipeline 3.4.3 “Elastine Durcie” |
| Filters | Proteins: log (e-value) < −5 Proteins: minimum 2 peptides Peptides: e-value <0.01 |
| Digestion | Trypsine No semi-tryptic peptide allowed 1 misscleavage allowed in first pass and 5 in refine pass |
| Modifications | Fixed Carbamidomethylation of Cys residues = +57.04 |
| Possible Oxidation of Met and Trp residues = +15.99 N-ter acetylation = +42.01 N-ter deamidation of Gln residues = −17.02 Nter deamidation on carbamidomethylated Cys = −17,02 Loss of H2O on N-ter Glu residues = −18.01 Deamidation of Gln and Asn residues = +0.98 N-ter Met excision |
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