DPPH radical scavenging activity (%) at 1 mg/ml and EC50 (mg/ml) of lipid classes obtained from chloroform/methanol and supercritical carbon dioxide extraction from Sargassum muticum according to the extraction method and DPPH radical scavenging activity (%) at 10 μg/ml and EC50 (μg/ml) obtained from BHA.
|Extraction method||Lipid class||Control|
|% Scavenging||EC50||% Scavenging||EC50||% Scavenging||EC50||% Scavenging||EC50|
|CHCl3/MeOH||23.6 ± 0.5a-α||> 5||29.6 ± 3.4a-β||4.1 ± 0.3||28.0 ± 1.2a-α, β||4.8 ± 0.1||83.3 ± 2.9||5.7 ± 0.3|
|sc-CO2||26.2 ± 1.2a-α||3.8 ± 0.5||50.9 ± 0.8b-β||0.9 ± 0.1||48.4 ± 1.6b-β||1.0 ± 0.1|
Values are the mean of three replicates (mean (% dw) ± s.d.). s.d., standard deviation. sc-CO2, supercritical carbon dioxide, BHA, butylated hydroxyanisole, EC50 refers to the quantity of extract required to reduce DPPH radical by 50%. a-b, letters in the same column represent significant differences according to Tukey’s pairwise a posteriori test, considering p < 0.05. α-β, letters in the same row represent significant differences according to Tukey’s pairwise a posteriori test, considering p < 0.05.
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