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Table 2

Mass distribution, density and chemical composition of the three liquid phases in the filtrate.

Standard Upper hydrophobic Hydrophilic Lower hydrophobic
phase phase phase

Mass distribution (%) 11.3 75.4 6.6
Density 0.972 ± 0.00 1.008 ± 0.00 1.030 ± 0.00
Chemical composition NF V 03-903 70.8 ± 0.1 97.6 ± 0.0 79.6 ± 0.1
Moisture (%)
Minerals (% of dry matter) NF V 03-922 1.9 ± 0.1 20.6 ± 0.1 6.5 ± 0.1
Lipids (% of dry matter) Demixing using ethanol/ether 77.3 ± 0.1 5.3 ± 0.5 60.2 ± 0.4
(3/1) (Mechling, 2002)1
Bligh and Dyer method (1959)2
Proteins (% of dry matter) NF V 18-100 15.0 ± 0.1 23.3 ± 0.0 15.0 ± 0.0
Pectic substances (% of dry matter) Blumenkrantz and Asboe- 0.6 ± 0.1 12.3 ± 0.3 9.7 ± 0.4
Hansen method (1973)
Non pectic sugars (% of dry matter) Colori metric method of 0.7 ± 0.0 8.7 ± 0.3 8.7 ± 0.2
Dubois et al. (1956)

1 The demixing method using ethanol/ether (3/1) was used for the upper and lower hydrophobic phases. 2 The Bligh and Dyer method was used for the hydrophilic phase.

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