Volume 13, Number 1, Janvier-Février 2006
|Page(s)||31 - 34|
|Published online||15 January 2006|
Les biomarqueurs de la peroxydation lipidique
UMR 585 Inserm/Insa de Lyon Laboratoire de physiopathologie des lipides et membranes Bât. Louis Pasteur
04 72 43 85 24
Different biomarkers related to lipid peroxidation are available to assess an oxidative stress. They are more or less specific and sensitive. For instance malondialdehyde (MDA) or conjugated diene are easily measured in different matrix. Their measurements provide only a rough indication assessing an oxidative stress. But sometimes information is wrong due to artefacts. Some markers such as isoprostanes are specific of arachidonic acid only, and they require expensive equipment such as GC-MS/MS. They are mainly dedicated to clinical investigation. We present here new markers of oxidative stress which allow to discriminate between the oxidation of polyunsaturated fatty acid families. They are 4-hydroxynonenal (4-HNE) and 4-hydroxy-hexenal (4-HHE) which represent the peroxidation of the n-6 and n-3 polyunsaturated fatty acids, respectively and 4-hydroxy-dodecadienal (4-HDDE) which derives from the breakdown of 12-HpETE and mainly reflects the 12 lipoxygenation of arachidonic acid. Those markers can be easily measured with a high sensitivity by GC-MS in plasma. Their urinary carboxylic oxidized end-products, namely 4-HNA, 4-HHA and 4-HDDA may also be measured by GC-MS. The choice between these different biomarkers is not easy. It depends on which information is required.
Key words: biomakers / lipid peroxidation / oxidative stress
© John Libbey Eurotext 2006
Current usage metrics show cumulative count of Article Views (full-text article views including HTML views, PDF and ePub downloads, according to the available data) and Abstracts Views on Vision4Press platform.
Data correspond to usage on the plateform after 2015. The current usage metrics is available 48-96 hours after online publication and is updated daily on week days.
Initial download of the metrics may take a while.