Volume 27, 2020
Technological challenges in oilseed crushing and refining / Défis technologiques de la trituration et du raffinage des oléagineux
|Number of page(s)||17|
|Published online||17 November 2020|
Figure S1. Chromatograms obtained after enzymatic hydrolysis of U-SFM with 30 nkat AnFaeB/g DDM (A) and 10 nkat ChlE/g DDM (B) at t = 0 min (red) and t = 240 min (black) at 326 nm. 3-O-caffeoylquinic acid (3-CQA) (1), 4-O-caffeoylquinic acid (4-CQA) (2), 5-O-caffeoylquinic acid (5-CQA) (3), caffeic acid (4), 5-O-coumaroylquinic acid (5-CoQA) (5), 5-O-feruloylquinic acid (5-FQA) (6), 3,5-Di-O-caffeoylquinic (3,5-diCQA) (7) and 4,5-Di-O-caffeoylquinic (3,5-diCQA) (4,5-diCQA) (8). From Laguna (2019).
Figure S2. Chromatograms obtained after enzymatic hydrolysis of U-RSM (A) and U-RSM dry methanolic extract (B) with 39 nkat AnFaeA/g DDM at t = 0 min (red) and t = 240 min (black) at 323 nm. Sinapoyl-kaempferol derivatives (1), sinapine (SNP) (2), trans-sinapic acid (SA) (3), cis-sinapic acid (SA) (4), di-sinapoyl derivatives (5), Tri-sinapoyl derivatives (6), sinapoyl glucose (7) and 1,2-di-O-sinapoyl-β-D-glucose (8). From Laguna (2019).
Figure S3. Chemical structure and NMR 1H and 13C data of 1,2-di-O-sinapoyl-b-D-glucose. From Laguna et al. (2019).
Figure S4. Chemical structure and LC-MS data of 1,2-di-O-sinapoyl-b-D-glucose. From Laguna et al. (2019).
Figure S5. Possible mechanism for the formation of 1,2-di-O-sinapoyl-b-D-glucose by transesterification of sinapine with 1-O-sinapoyl-b-D-glucose catalyzed by AnFaeA. Note: Mechanism adapted from Dilokpimol et al. (2016).(Access here)
© O. Laguna et al., Hosted by EDP Sciences, 2020
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