AKG supplementation partially restores the plasmalogen and fatty acid profile of the pups plasmalogen-deficient retina. A) Plasmalogen deficient (DAPAT^−/−) mice retina were completely devoid of plasmalogens, while they represent 6% of total phospholipids in WT mice retina. 3 weeks of alkylglycerol-enriched shark liver oil (plasmalogen precursor) nutritional supplementation through lactation was able to significantly increase plasmalogen levels in the retina of plasmalogen deficient pups (DAPAT^−/− + AKG), but remained significantly lower than in WT mice. B) Plasmalogen molecular species. The main plasmalogen species are saturated. Interestingly, SLO maternal supplementation increased the concentration of 18:1n−7 but not those of 16:0, 18:0 and 18:1n−7 plasmalogens. C) Fatty acid profile of the retina. Plasmalogen deficiency was associated with a significant increase in saturated fatty acids (SFA) and monounsaturated fatty acids (MUFAs), while polyunsaturated fatty acids (PUFAs) were significantly decreased. SLO maternal supplementation partially restored SFA and PUFA concentrations. * p <0.05; ** p <0.01; ***p <0.001. n = 4–8. One-way ANOVA with post-hoc Tukey’s multiple comparisons test. FAMEs: Fatty acid methyl esters; DMAs: Dimethylacetals.