The PHENOLEO project or how to separate and add-value to phenolic compounds present in rapeseed and sunflower meals | OCL

Technological challenges in oilseed crushing and refining / Défis technologiques de la trituration et du raffinage des oléagineux

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Table 6

Maximal amount and yield of caffeic acid (CA) after enzymatic treatment of T-SFM and U-SFM dry methanolic extract with ChlE and AnFaeB (from Laguna et al., 2019).

Sample	Enzyme	Initial TCAD^* in the meal (μmol CAE/g DDM)	TCAD at t = 0 of enzyme incubation^§ (μmol CAE/g DDM)	Maximum CA released after incubation with enzyme (μmol/g DDM)	Incubation time (min)	CA yield (%) based on TCAD at t = 0 of enzyme incubation^§
T-SFM	ChlE	57.4 ± 1.5	59.9 ± 1.7	54.0 ± 1.1	240	90
T-SFM	AnFaeB	57.4 ± 1.5	59.9 ± 1.7	59.8 ± 2.1	180	100
U-SFM extract	ChlE	71.7 ± 0.5	66.5 ± 2.0	55.4 ± 0.3	240	77
U-SFM extract	AnFaeB	71.7 ± 0.5	66.5 ± 2.0	68.1 ± 1.7	10	95

Note: *: TCAD = total caffeic acid derivatives; CAE = caffeic acid equivalent. DDM = defatted dry matter. Values are mean ± SD (n = 2). §: The substrate was pre-incubated for 30 min in MOPS buffer at 50 °C before adding enzyme (t = 0 of enzyme incubation).

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