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Table 5

Comparison of direct and indirect method of 3-MCPD esters and glycidol esters analysis.

Direct methods Indirect methods

Pros No chemical reaction Standards available
Individual quantification of species Good sensitivity

Cons Standards not available (60–70 different species) Reactivity of the analytes (glycidol and 3-MCPD)
Expensive tools: HPLC/MS/MS or HPLC/TOF/MS Global quantification of glycidyl esters and 3-MCPD
Purification step needed esters

Papers Masukawa et al., (2010) Divinová et al.,
Masukawa et al., (2011) (2004)
AOCS Cd 28–10(1) Weißhaar, (2008)
Haines et al., (2011) DGF C-VI 18 (2010)(2)
Dubois et al., (2011) Hrncirik et al., (2011)
Dubois et al., (2012) Kuhlmann, (2011)
Moravcova et al., (2012) Ermacora et al., (2012)
1.

The American Oil Chemists’ Society (AOCS) and the Japan Oil Chemists’ Society (JOCS) released the first validated direct method for glycidyl fatty acid esters in edible oils, AOCS/JOCS Official Method Cd 28–10.

2.

Deutsche Gesellschaft für Fettwissenschaft (DGF) evaluated a method for the determination of the sum of ester bound 3-MCPD and Glycidol.

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