Open Access
Table 2
Chromatographic conditions of the RP-DAD-HPLC method.
| Method parameters | HPLC3/DAD-HPLC |
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| HPLC-device | Merck Hitachi, (VWR LaChrome Elite), pump L-2130; |
| autosampler L-2200 | |
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| Data system | EZChrom Elite Version 3.3.2 SP2 Build 3.3.2 1037 |
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| HPLC column: | Lichrospher®> 100 RP-18e (5 μm), 250-4 mm |
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| Pre column: | Lichrospher® 100 RP-18e (5 μm), 4-4 mm |
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| Eluent: | Eluent A: Water acidified with o-Phosphoric Acid |
| (pH 2,3−2,6); eluent B: methanol (gradient grade) | |
| Eluent B: Methanol (HPLC gradient grade) | |
| Eluent: C: CAN:THF (4:1) (v:v) | |
| Eluent profil (v/v): | ||||
| min | A [%] | B [%] | Flow [ml/min] | |
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| 0.00 | 70 | 30 | 1.0 | |
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| 5.00 | 70 | 30 | 1.0 | |
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| 15.00 | 30 | 70 | 1.0 | |
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| 20.00 | 10 | 90 | 1.0 | |
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| 23.00 | 10 | 90 | 1.0 | |
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| 23.10 | 0 | 100 | 1.0 | |
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| 25.00 | 0 | 100 | 1.0 | |
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| 25.10 | 70 | 30 | 1.0 | |
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| 30.00 | 30 | 70 | 1.0 | |
| Detector (wavelenght): | DAD-detektor Hitachi L-2455, Channels: λ1 = 280 nm, |
| λ2 = 325 nm | |
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| Column temperature: | Column oven VWR Hitachi L-2350 (35 °C) |
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| Injection volume: | Standard: 10 μl Probe: 10 μl |
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| Method name | Sinapinsäure_Canolol.met |
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