Issue |
OCL
Volume 27, 2020
|
|
---|---|---|
Article Number | 45 | |
Number of page(s) | 8 | |
Section | Agronomy | |
DOI | https://doi.org/10.1051/ocl/2020041 | |
Published online | 10 September 2020 |
Research Article
Doubled haploids of interspecific hybrids between Brassica napus and Brassica rapa for canola production with valuable breeding traits
Haploïdes doublés d’hybrides interspécifiques entre Brassica napus et Brassica rapa pour la production de colza avec des caractéristiques de sélection avantageuses
Laboratory of Breeding and Biotechnology, Institute of Plant Biology and Biotechnology,
45, Timiryazev str.,
050040
Almaty, Kazakhstan
* Correspondence: zhambakin@gmail.com
Received:
12
March
2020
Accepted:
14
July
2020
Doubled haploids (DH) were obtained from two interspecific hybrids between Brassica napus and Brassica rapa. Seeds of doubled haploid plants differed in colour and size. The hybridity of the obtained doubled haploid is shown using genomic in situ hybridization (GISH) analysis. Evaluation of drought tolerance during seed germination on PEG-6000 showed the advantage of doubled haploid plants of interspecific hybrids over the parent cultivars. The oil from seeds of doubled haploid plants showed good nutritional value.
Résumé
Des haploïdes doublés (DH) ont été obtenus à partir d’hybrides interspécifiques entre Brassica napus et Brassica rapa. Les graines de ces plantes haploïdes doublés différaient en couleur et en taille. L’hybridité de l’haploïde doublé obtenu est démontrée par une analyse génomique par hybridation in situ (GISH). L’évaluation de la tolérance à la sécheresse pendant la germination des graines sur PEG-6000 a montré l’avantage des plantes doubles haploïdes issues d’hybrides interspécifiques par rapport aux cultivars parents. L’huile des graines de plantes doublement haploïdes a montré une bonne valeur nutritionnelle.
Key words: Brassica napus / Brassica rapa / interspecific hybrid / doubled haploid / drought tolerance
Mots clés : Brassica napus / Brassica rapa / hybride interspécifique / haploïde doublé / tolérance à la sécheresse
© A. Daurova et al., Hosted by EDP Sciences, 2020
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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