Volume 11, Number 6, Novembre-Décembre 2004
|Page(s)||464 - 468|
|Published online||15 November 2004|
Activity and stability of immobilized lipases in lipase-catalyzed modification of peanut oil
Research Laboratory for Applied Chemistry and Biology (LARECBA), Abomey-Calavi Polytechnic School, Abomey-Calavi University, 01 BP 2009, Cotonou, Benin
2 Faculty of Sciences and Technics, Department of Biochemistry and Cellular Biology, BP 526, Cotonou, Benin
3 Institute of Chemistry and Biochemistry, Department of Technical Chemistry and Biotechnology, University Greifswald, Soldmannstr, 16, D-17487 Greifswald, Germany
Accepted: 17 January 2005
Fatty acid release during lipolysis of peanut oil using microbial free and immobilized lipases in aqueous media was developed. Immobilized lipase from Rhizomucor miehei (RML) gave the best result from its ability to clive different fatty acids from peanut oil in such media. In organic solvent, interesterification of peanut oil with tricaprylin using immobilized lipases from RML, Chromobacterium viscosum (CVL) and Candida rugosa (CRL) was performed. The best substrate molar ratio of tricaprylin to peanut oil found was in the range 0.7 to 0.8. Using substrate molar ratio 0.7, high amount of structured triglyceride ST (about 35% MLM, 44% LML triglyceride fractions) was obtained with lipase from RML in n-hexane. The results found in solvent free system were in some cases quite similar to that obtained in organic solvent. In nine successive batch interesterification in solvent free medium using immobilized RML and CRL, no significant loss of amount of both produced triacylglycerol fractions until batch 7 was observed with RML.
Key words: interesterification reaction / peanut oil / microbial lipases / immobilization / structured triacylglycerols / stability
© John Libbey Eurotext 2004
Current usage metrics show cumulative count of Article Views (full-text article views including HTML views, PDF and ePub downloads, according to the available data) and Abstracts Views on Vision4Press platform.
Data correspond to usage on the plateform after 2015. The current usage metrics is available 48-96 hours after online publication and is updated daily on week days.
Initial download of the metrics may take a while.