Table 7

Maximal amount and yield of sinapic acid (SA) after enzymatic treatment of T-RSM, U-RSM and U-RSM dry methanolic extract with AnFaeA (from Laguna et al., 2019).

Sample Initial TSAD* in the meal (μmol SAE/g DDM) TSAD at t = 0 of enzyme incubation§ (μmol SAE/g DDM) Maximum SA released after 240 min incubation with enzyme (μmol/g DDM) SA yield (%) based on TCAD at t = 0 of enzyme incubation§
T-RSM 46.0 ± 0.3 44.8 ± 0.2 31.6 ± 0.1 70
U-RSM 64.2 ± 1.2 53.1 ± 1.0 41.3 ± 0.3 78
U-RSM extract 59.2 ± 2.3 32.3 ± 0.4 55

Note: *: TSAD = total sinapic acid derivatives; SAE = sinapic acid equivalent. DDM = defatted dry matter. Values are mean ± SD (n = 2). §: The substrate was pre-incubated for 30 min in MOPS buffer at 55 °C before adding enzyme (t = 0 of enzyme incubation).

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