Evaluation of green extraction methods on the chemical and nutritional aspects of roselle seed (Hibiscus sabdariffa L.) oil | OCL

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Table 4

Total phenolic content, antioxidant activity and oxidative stability of RSO samples.

Parameter	Extraction methods

	Supercritical (CO₂)	Hydraulic-press	Screw-press	Solvent
Total phenolic content (mg of GAE/g)	22.18 ± 1.13^a	17.25 ± 1.07^b	13.74 ± 0.55^c	18.10 ± 1.43^b
DPPH radical-scavenging activity (%)	65.47 ± 2.08^a	57.52 ± 1.33^b	49.59 ± 0.74^c	55.67 ± 1.09^b
EC₅₀ (mg/mL)	0.507^d	0.577^c	0.661^a	0.593^b

EC₅₀ value, the effective concentration at which the antioxidant activity was 50% and it was obtained by extrapolation from linear regression analysis; data are expressed as mean ± standard deviation of three determinations.

Values in the rows with different letters (a–d) are significantly different (p < 0.05). The same letters at the same column are not significant (p < 0.05).

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