Semi-pilot scale-up of a continuous packed-bed bioreactor system developed for the lipase-catalyzed production of pseudo-ceramides | OCL

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Table 1

Comparison of efficiency between the laboratory and semi-pilot scales of the continuous packed-bed bioreactor system.

Bioreactor scale	Step	Operational parameters						Yield (%)	Production rate (g h^-1)

		Flow rate (ml min^-1)	Residence time (min)	Biocatalyst amount (g)	[Fatty acid^b] (mM)	[Acyl acceptor^c] (mM)	Packed-bed (diameter/length, cm)
Laboratory^a	1	0.5	5.7	0.875	100	100	14.5/0.5	92	0.99
Laboratory^a	2	0.25	11.4	0.875	150	50	14.5/0.5	54	0.23
Semi-pilot	1	2.5	11.6	10	100	100	1.5/5	86	4.60
Semi-pilot	2	2.5	11.6	10	150	50	1.5/5	25	1.10

From Le Joubioux et al. (2014).

Stearic acid 2d in step 1 and myristic acid 2b in step 2.

3-amino-1,2-propanediol 1 in step 1 and N-stearyl 3-amino-1,2-propanediol 3d in step 2.

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