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Activity evolution and number of active recording channels for a cortical culture in guar gum in the perfusion system at ambient air over a period of 55 DIVs. Each dot represents the cumulative activity (sum of recorded action potentials) during one minute time bins on all active channels (blue trace). Each day is depicted with a different dot color. Buffer types are color-coded along the x-axis. The perfusion configuration is sketched below the graph.
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