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Effect of DHA-Alb on permanent MCAo. (a) Total neurological score (normal score = 0, maximal score = 12) during MCAo (60 min) and on days 1, 2 and 3 after treatment; (b) total infarct volume and (c) computer-generated MosaiX-processed images of Nissl stained paraffin-embedded brain sections on day 3 after stroke. Treatment with DHA-Alb significantly improved neurological scores (48 and 72 h), showed less extensive damage, mostly in the subcortical area and reduced total lesion volumes compared to the corresponding Alb-treated group. Values shown are means ± SEM. #p< 0.05versus saline group; p< 0.05versus Alb (1.25 g/kg) group (two-way repeated-measures ANOVA).

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