Enzymes for industrial applications

This is anOp Abstract – Protéus is a biotechnology company specializing in the discovery, engineering and production of enzymes for industrial applications, as well as in the development of innovative bioprocesses involving these enzymes. Protéus is a subsidiary of the PCAS Group, actor in fine chemicals and specialty products and producer of high-value complex molecules. Enzymes allow considering unique functionalizations that are difficult to achieve by conventional chemical means. Examples involving the screening of our ready to use toolbox of lipases, the engineering of the well-known lipase CalB and the specific modifications of lipids will be presented.

Enzymes are considered to be little miracle substances for innovation, process advantages and cost reductions. Ok, it's not magic but "hard science" -nonetheless, the performance of enzymes in some applications is really breathtaking, especially compared to "conventional" chemical synthesis routes. Whether in the food industry, pharmaceutical industry, textiles, detergents, bio refineries, fine and specialty chemicals, paper and cosmetics, the application areas in which enzymes can be used sensibly and profitably are diverse and nowhere near exhausted. As research and development advance and ever more processes make it into industrial applications, some trends emerge that might lead the way for the development of the industry. The DECHEMA PRAXISforum "Enzymes for Industrial Applications" brings together top-class industry professionals, solution providers and key customers from over 65 companies in the field of industrial enzymes from all over the world. In this PRAXISforum they have the opportunity to exchange and network with colleagues from other companies and branches, with potential cooperation partners, and get informed about the latest trends, innovations and practices. At the same time participants can present their needs and future requirements in direct exchange with suppliers of technologies, services and products.

What the PRAXISfourm is about
• By industry, for industry -the PRAXISforum reveals market opportunities and promotes the development of new enzymes, production processes and application areas. • Networking platform -the PRAXISforum brings together international market leaders and high profile end-users and experts from all relevant industries. • High-level speakers -best practices presentations and lessons learned from speakers at decision-maker level. Technological background is as much presented as end-user relevant information. • Relevance to applications -the PRAXISforum provides visitors an overview on innovations for their highly specific requirements of everyday practice.

Topic tables
At the topic tables you can discuss specific questions, developments, challenges and their solutions with our speakers. In every break you can find the experts from the prior session at an indicated topic table within the exhibition area. Are you just getting started with industrial enzymes? You want to discuss your question in a smaller group? You don't agree with the statement given by the speaker? -Make your way to our topic tables! Topic

Networking Dinner
At the evening of the first PRAXISforum day we continue the discussions about trends, developments, new technologies and products in the field of industrial enzymes within a relaxed and informal networking dinner.
To set a new stimulus for interesting discussions and networking and to fresh up your minds we will change the location and move to the Life-Style bar at the Maritim hotel, close to one of Frankfurt's famous landmark buildings, the MesseTurm. It's a perfect place for relaxed networking.

The Speakers
Kai Baldenius Head of Biocatalysis Research, BASF / Germany "We haven't fully appreciated the potency of enzyme catalysis, let's move out of the chiral niche and target chemical production more broadly" Kai Baldenius is a chemist by formation. After receiving a PhD from Hamburg University, he spent a post-doc research year at The Scripps Research Institute before he joined BASF in 1993. In BASF Kai started his career in the central research doing process development work for vitamins and other fine chemicals. He took over responsibilities in production, marketing and sales, before he returned to research. Since 2009, Kai heads the BASF biocatalysis research group.

Yasuhisa Asano
Head of Enzyme Chemistry Laboratory, Toyama Prefectural University, Biotechnology Research Center / Japan "Active enzymes: Actively expressed enzymes in heterologous hosts are always necessary. Enzyme activity: Activity measurement is important in enzyme discovery. Enzyme molecule: Enzymes are improved by looking at protein structure in molecular level."

Sven Benson
Co-founder & CEO, Candidum / Germany "One of the major bottlenecks to making biocatalysts economically viable is to find the right solutions for specific catalytic challenges within the vast diversity of enzyme blueprints. candidum sets out to drive innovation by making this process simple, budgetable, at competitive costs and with minimal risks." Sven Benson studied biotechnology at the University of Stuttgart, where he was awarded his PhD from the SimTech Cluster of Excellence with focus on molecular enzyme modeling. He stayed on as a research fellow to finish his work on parts of candidum's core bioinformatics technologies. He co-founded candidum where he currently acts as CEO.

Fredi Brühlmann
Group leader Biotechnology Department, Firmenich / Switzerland "Enzymes offer highly versatile chemistries under benign conditions enabling diverse applications. While an increasing range of engineering options helps addressing performance and cost issues among others, it is important that the regulatory environment and the public remain open to science and technology." Andrea Dreusch CEO, MicroMol / Germany "Phages are much more than a redoubtable contamination. If you deal with a good risk management they might be friends. There are much more possibilities to talk about bacteriophages than only to execrate them" Mrs. Dreusch studied Biology with focus in Microbiology to get a Dipl. Biol. and continued working in the field of Molecular Biology (PhD). In 1996 she founded the MicroMol Laboratory together with her husband and worked as Co-CEO developing her company in the main biotechnological fields. Well-known as an interface laboratory covering microbiology, molecular biology, cell biology and immunology MicroMol established the interesting core-competence of Bacteriophage works.
After a short stopover working as Director of BAV-Academy, Mrs. Dreusch returned to MicroMol when her husband suddenly died in 2017 to accept responsibility for MicroMol again.
She integrated the laboratory into the Tentamus group, which offers a global network of highly specialized laboratories, all of which are equipped to ensure the quality and safety of the tested goods.  His current studies focus on the re-design of API and intermediated synthesis to allow an extensive use of the biocatalysis. He is also involved in all the issues related to the use and stabilization of enzymes in industrial implementation. These works, developed in collaboration with University of Padova where dr. Motterle is responsible for a PhD and a PostDoc on Industrial Biotechnology, are object of some publications and patents and were presented at several scientific conferences.

The Speakers
Jörg Ohl Senior Business Development Manager, Corden BioChem / Germany "Contract Manufacturing becomes more and more important in biotechnology. For strategical as well as tactical outsourcing, a reliable and experienced partner delivering on-time and in the right quality is a must" • 7+ years experience in manufacturing operations, FDA and GMP compliance, bulk manufacturing and production management

Audrey Robic
Scientific Manager, Protéus / France "To increase the chances of success in finding the best biocatalyst and process conditions for your applications, it is important to access panels of biocatalysts ready for screening and a streamlined methodology to assess rate-limiting process parameters" Audrey has a dual training in pharmacy and biochemistry. She is a Scientific Manager at Protéus. She manages R&D teams and projects. These projects concern biocatalyst screenings, biocatalyst optimizations from lab to industrial scale for pharmaceuticals and cosmetic ingredients. Before joining Protéus, Audrey has held several project manager positions in the biotechnology units of feed and food companies.

Alexander Pelzer
Platform Coordinator Tailor-made Biocatalysts, BRAIN / Germany "Enzyme product development is a multidisciplinary task that requires the combination of excellence in diverse fields like enzyme discovery and identification, microbial enzyme production and formulation of the enzyme in an application compatible formula with long storage stability." Dr. Alexander Pelzer studied biology at Heinrich-Heine-Universität Düsseldorf. During his doctoral studies at the Institute of Molecular Enzyme Technology at Jülich Research Centre, he worked on the production of various enzymes derived from pseudomonads, as well as on their biochemical and physiological characterisation. Pelzer has been working at BRAIN as a research scientist and project manager in the Unit Enzyme Technologies since 2014. In 2017 Pelzer became leader of the technology platform Tailor-made Biocatalysts dealing with application directed enzyme identification and optimization.

Wojtek Steffen
Project Leader Enzyme and Protein Technologies, Roche Diagnostics GmbH / Germany "Conventional chemical strategies for protein conjugation are often lacking in site-specificity, linkage stability and reaction conditions. This effected the development of biocatalysts that confer regioselectivity for site-specific modification. Microbial Transglutaminases have long been known as promiscuously 'protein-glueing' enzymes in food technology, but are now emerging as highly specific, versatile and inexpensive tools for a broad range of industrial applications."

The Speakers P R A X I S fo r um E N Z YM ES F O R I N D U ST R I A L A P P L I C AT I O N S • 0 6 -07 Fe b 2 0 1 8
The exhibition is an integral part of the PRAXISforum. It is a platform for the latest product and technology innovations and manufacturing processes. As a visitor, you are invited to take a really close look at every exhibit.
Please find more information about our exhibitors in their company profiles on the next pages.
The following companies are looking forward to your visit and will inform you about the latest trends and decisive advantages of their products and services to you and your customers.
Amicogen is a global leading company in specialty enzymes. The word "Amicogen" consists of the meaning of friend, "AMICO" in Latin, and of gene, "GENE". We are a "Bioengineering company just like a Sincere Friend" who contributes to the human health and the environment. We have developed many innovative biocatalysts by applying our proprietary i DNA Evolution™ technology. We developed the world's first cephalosporin C (CPC) acylase for the production of 7-aminocephalosporin C (7-ACA) utilizing one-step enzyme process. We keep ourselves one step ahead to expand our enzyme business into the pharmaceutical, food/feed, environment, and bioenergy industry. We can provide our R&D expertise, manufacturing facility, and innovative enzyme products for you. We positively welcome your any business or technology proposal and hope to create many innovative products and success stories which can change the world together with you.

Biokal
Biokal represents Resindion s.r.l. / A subsidiary of Mitsubishi Chemical Corporation, Bio-Works Ltd. and Eurodia Industrie SA. We are offering solutions for preparative and industrial scale chromatography, purification processes and biocatalysis applications. Our products cover a range of ReliZyme™ enzyme carriers for immobilized enzyme applications, ReliSorb™ industrial chromatography media, Diaion™ ion-exchange and adsorbent resins and, WorkBeads™ agarose based chromatography media. Our services include technical and scientific application support starting from lab-bench up to industrial realization. Being located in the Netherlands we serve the Benelux countries, Germany and Denmark with our products and services.

BlueSens gas sensor GmbH
BlueSens is your specialist for gas analysis and bioprocess software, focused on bioprocesses from lab to industrial scales.
Founded in 2001 we have more than 15 years of experience and are market leader in gas analysis solutions for all kinds of bioprocesses. Our philosophy is to measure it where it happens when it happens. With our high quality sensors we serve our customers from all over the world working in pharma, food, feed as well as biogas sector.
Our customers from enzyme production and development use the off-gas analysis for process optimization and scale-up tasks. Recently launched BlueVary maintenance free off-gas sensor allows adaption to changing requirements while avoiding downtimes.
All BlueSens´ sensors work independent from scale and are compatible with the most common bioreactor types. It´s our objective to offer our customer best quality, an appealing price/performance ratio and the best possible service.
No matter if you need process sensors (O2, CO2, CH4, H2, EtOH), complete systems (eg Yieldmaster) or software (BlueVis) we assist you in finding the right solution. All sensors are produced in Germany following our strictest quality guidelines. Do you have questions or try to solve an untypical problem? Contact us we are well known for our excellent customer service.

EnginZyme AB
In spite of the many benefits of biocatalysis, the potential still far exceeds the current exploitation. At EnginZyme, a Swedish biocatalysis company founded in 2014, we're on a mission to change this. The main obstacle for biocatalysis to go mainstream remains the cost of use; in many cases enzymes simply cannot compete with traditional chemical alternatives.
To solve this problem, we have developed EziG, a general enzyme immobilization carrier specifically designed for biocatalysis. Any His-tagged enzyme can be bound to an EziG-carrier in a non-destructive, fast and selective way, enabling enzyme reuse without loss of activity. The material functions in both aqueous and organic solvents, and is perfectly suited for both batch and flow chemistry applications.
Armed with this new tool, we look forward to doing our part in helping the industry transition to a more sustainable way of operating.

Enzymicals AG
The focus of Enzymicals is to provide integrated solutions for biocatalytic applications. Dedicated to design, develop and implement cost-effective, sustainable and scalable chemo-biocatalytic routes, Enzymicals offers a recognized expertise in the application of enzymatic processes for the synthesis of complex chemicals.
• The independent enterprise provides state of the art SOLUTIONS in development, optimization and scale-up of robust chemo-enzymatic processes for the production of fine & specialty chemicals.
• The company manufactures a range of CHEMICALS as chiral building blocks, intermediates and specialty chemicals as well offer custom synthesis on request.
• Enzymicals offers a broad selection of recombinant ENZYMES suitable for research, development, production and diagnostics as well as a tailor-made protein expression and optimization service.
Enzymicals service covers the whole value chain towards a tailor-made process for a special application: enzyme identification, expression, characterization, improvement and application in an efficient production process. Together with our network partners, we expand our service to cGMP production and bulk scale supply.

Dr. Ute Liebelt Projekt Manager Sales and Marketing
By exploiting the strong synergies in bioreactor technology and polymer manufacturing, Eppendorf has emerged as a global player and valuable resource to its customers in the bioprocess marketplace. With a comprehensive offering of single-use and traditional products for the growth of mammalian, microbial, insect, plant and algae cells, and working volumes of 60 mL -1,200 L, the Eppendorf bioprocess portfolio can satisfy the demands of process development through production. From the parallel mini bioreactor system for early stage bioprocess development, the benchtop and parallel bioreactor systems for the laboratory scale to the sterilize-in-place solutions for production: Eppendorf offers users from industry and research extensive bioprocess solutions from a single source and meets the highest quality demands.
Seamlessly progress from R&D to large scale production -all from one source: Eppendorf. GenoFocus is a platform technology company developing enzyme solutions for specialty food, eco-friendly processes, biopharmaceuticals, cosmeceuticals and agriculture. We develop enzymes through molecular evolution using smart library generation and microbial display-based high throughput screening. With fungal and bacterial secretion systems, we custom produce the evolved enzymes in large scale fermentation and downstream processes.

GlycoSpot
GlycoSpot supplies efficient enzyme screening kits for industries such as enzyme discovery, food and feed, detergent and bioenergy. Our ready-to-use standard kits (with more than 30 pure and complex substrates to choose from) will provide you with conclusive information about the present enzyme activity in less than 30 minutes hands-on-time. GlycoSpot's customized biomass substrates, produced from raw biological material, add an additional unique dimension to information gathered about a specific enzyme's activity and can be used to screen complex biomass in its original format.
Our focus is on your needs for enzyme screening solution. Please do not hesitate to contact us, we customize assay designs and substrates, and we make substrates out of almost anything! INOFEA is a Swiss biotech company founded in 2014 in Basel as a spin-off from the School of Life Sciences, University of Applied Sciences and Arts, Northwestern Switzerland (FHNW).
At INOFEA, we empower enzymes. Together with our team of scientists led by Dr Patrick Shahgaldian, CSO of INOFEA, and with the help of our investors, we have developed enzzen©, a patented platform technology capable to fit enzymes to in vivo and process conditions. We immobilize the enzymes of interest onto safe silica particles and we further grow a custom-designed protection layer. This provides the enzyme with remarkable performance.
We've created INOFEA from the desire to meet a critical need related to enzyme lack of stability and robustness: the possibility to provide enzymes with resistance to in vivo and process conditions, so that they could be fully deployed in the health industry.
Unlike other existing technologies, our innovative nanotechnology platform is capable of shielding sensitive enzymes and has positively demonstrated substantial benefits to customers in a number of biopharma-related applications.
INOFEA is constantly investing in R&D for its proprietary technology, as well as diversifying its line of business with value-added products and services, from formulating new commercially available enzymes, to improving our client's processes by embedding enzzen© to non-proprietary applications.

Empowering Enzymes
The generic design of the SpinChem ® RBR allows for seamless scalability, whereby the technology can be utilized with liquid volumes from 10 mL up to several thousand cubic meters. Where required, customer specific features can be incorporated and SpinChem ® can offer help with process development, all the way from bench-top screening to full-scale production.
SpinChem ® AB is a technology company working at the boundaries of physics, chemistry and biotechnology whose products have applications in biotransformation, downstream processing, cleantech and food & ingredients.
Through the development of the rotating bed reactor (RBR), SpinChem ® is able to provide dramatic improvements in the mass transfer characteristics of heterogeneous reactions involving a solid and a liquid phase.
The SpinChem ® RBR is a revolutionary concept that moves solutions through a packed bed of solid phase material in a manner that maximizes contact between the liquids and the solid phase particles, while still preserving the integrity and structure of the materials. SpinChem ® AB is a privately held company based in Umeå, Sweden whose clients include several of the largest companies working with fine chemicals, pharmaceuticals (biotransformation) and industrial wastewater clean-up (cleantech).

VBU
The Association of German Biotechnology Companies (Vereinigung Deutscher Biotechnologie-Unternehmen, VBU) is a group of companies and institutions involved in biotechnology and related sectors. Founded in 1996, VBU was the first organization for biotechnology companies in Germany and is integrated in the Biotechnology Section of DECHEMA. Our members come from the fields of biotechnology, pharmaceutical technology, bioinformatics, diagnostics, medical devices and laboratory technology. VBU is a platform for cooperation, communication and information.

What we do
VBU is a catalyst for the transfer of research results into competitive products and services. It represents a cross-sectoral network, assisting its members in seeking and finding cooperation partners in all areas of the life sciences both on a national and an international scale.

What we offer
• Webinar series • Scientific committees for interdisciplinary cooperation and discrete exchange of information • Joint stands at national and international events • Partnering events • Contacts to graduates, postgraduates and students • Quaterly newsletter • Business network for women managers in the life sciences (more than 200 members) Wiley is a global provider of content-enabled solutions that improve outcomes in research, education, and professional practice. Our core businesses produce scientific, technical, medical, and scholarly journals, reference works, books, database services, and advertising; professional books, subscription products, certification and training services and online applications; and education content and services including integrated online teaching and learning resources for undergraduate and graduate students and lifelong learners.

ZYMVOl BIOMODELING
ZYMVOL BIOMODELING is a start-up company that uses computer simulations to accelerate the discovery and design of new industrial enzymes.
ZYMVOL has developed algorithms that can replace thousands of experiments with fast, accurate and cost-effective computer simulations. These allow you to center design efforts in the part of the enzyme that really matters which significantly reduces time, resources and money in each new biocatalysts development.
Our computational pipeline named ZYMEVOLVER combines full protein dynamics, explicit water representation and quantum mechanics. Our data-driven simulations account for realistic modeling (at low computational cost) making our in silico predictions far superior to all existing alternatives in the market. We will design your new enzyme in less than one month.
Advantages of working with ZYMVOL: Save time and money in your enzyme development Expand the application range of your enzyme portfolio Rescue dead-end projects Retain full IP of your enzymes Find suitable enzymes for a given molecule/reaction